Construct: ORF TRCN0000491946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000162.1_s317c1
- Derived from:
- ccsbBroadEn_10944
- DNA Barcode:
- AGACCAAAGTCAGACAGCACCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LASP1 (3927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NM_006148.4 | 72.6% | 58.2% | (many diffs) |
| 2 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NM_001271608.1 | 55.7% | 35.9% | (many diffs) |
| 3 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NR_073384.2 | 23.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccccaactgc gcccggtgcg gcaagatcgt gtatcccacg gagaaggtga 121 actgtctgga taagttctgg cataaagcat gcttccattg cgagacctgc aagatgacac 181 tgaacatgaa gaactacaag ggctacgaga agaagcccta ctgcaacgca cactacccca 241 agcagtcctt caccatggtg gcggacaccc cggaaaacct tcgcctcaag caacagagtg 301 agctccagag tcaggtgcgc tacaaggagg agtttgagaa gaacaagggc aaaggtttca 361 gcgtagtggc agacacgccc gagctccaga gaatcaagaa gacccaggac cagatcagta 421 acataaaata ccatgaggag tttgagaaga gccgcatggg ccctagcggg ggcgagggca 481 tggagccaga gcgtcgggat tcgcaggacg gcagcagcta ccggcggccc ctggagcagc 541 agcagcctca ccacatcccg accagtgccc cggtttacca gcagccccag cagcagccgg 601 tggcccagtc ctatggtggc tacaaggagc ctgcagcccc agtctccata cagcgcagcg 661 cccccatctg tcTTCAGCAC ATTCCACGGC ATCGCATCCG TCCTGGGCGT GACCCGTCCA 721 TTCTTCAGTG TCTCTGTTTT TTAAAACCTG CGACAGCTTG TGATTCCTAC CCCTCTTCCA 781 GCTTCTTTTG CCAACTGAAG CCTTCTTCTG CCACTTCTGC GGGCTCCCTC CTCTGGCAGG 841 CTTCCCCCTT GATCGACTTC TTGGTTTTCT CTCTGGATGG AACGGGCATG GGCCTCTCTG 901 GGGGAGGGCG AGGCCCGTGG GGCAGGGCTG GAATGGGAGA CCTGTTGGCC TGTGGGCCTC 961 ACCTGCCCCT CTGTTCTCTC CCCTCACATC CTCCTGCCCA GCTCCTCACA TACCCACACA 1021 TTCCAGGGCT GGGGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGACCAAAGT CAGACAGCAC 1201 CCTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt