Construct: ORF TRCN0000491953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020990.2_s317c1
- DNA Barcode:
- ACACTCGCATTCTAACTTGCCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NPY2R (4887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4887 | NPY2R | neuropeptide Y receptor Y2 | NM_000910.4 | 99.8% | 100% | 585C>T;936C>T |
| 2 | human | 4887 | NPY2R | neuropeptide Y receptor Y2 | NM_001370180.1 | 99.8% | 100% | 585C>T;936C>T |
| 3 | human | 4887 | NPY2R | neuropeptide Y receptor Y2 | XM_005263033.4 | 99.8% | 100% | 585C>T;936C>T |
| 4 | mouse | 18167 | Npy2r | neuropeptide Y receptor Y2 | NM_008731.3 | 87.5% | 94.2% | (many diffs) |
| 5 | mouse | 18167 | Npy2r | neuropeptide Y receptor Y2 | XM_006501115.1 | 87.5% | 94.2% | (many diffs) |
| 6 | mouse | 18167 | Npy2r | neuropeptide Y receptor Y2 | XM_006501116.1 | 87.5% | 94.2% | (many diffs) |
| 7 | mouse | 18167 | Npy2r | neuropeptide Y receptor Y2 | NM_001205099.1 | 61.2% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1215
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtcca ataggtgcag aggctgatga gaaccagaca gtggaagaaa 121 tgaaggtgga acaatacggg ccacaaacaa ctcctagagg tgaactggtc cctgaccctg 181 agccagagct tatagatagt accaagctga ttgaggtaca agttgttctc atattggcct 241 actgctccat catcttgctt ggggtaattg gcaactcctt ggtgatccat gtggtgatca 301 aattcaagag catgcgcaca gtaaccaact ttttcattgc caatctggct gtggcagatc 361 ttttggtgaa cactctgtgt ctaccgttca ctcttaccta taccttaatg ggggagtgga 421 aaatgggtcc tgtcctgtgc cacctggtgc cctatgccca gggcctggca gtacaagtat 481 ccacaatcac cttgacagta attgccctgg accggcacag gtgcatcgtc taccacctag 541 agagcaagat ctccaagcga atcagcttcc tgattattgg cttggcctgg ggcatcagtg 601 ccctgctggc aagtcccctg gccatcttcc gggagtattc gctgattgag atcattccgg 661 actttgagat tgtggcctgt actgaaaagt ggcctggcga ggagaagagc atctatggca 721 ctgtctatag tctttcttcc ttgttgatct tgtatgtttt gcctctgggc attatatcat 781 tttcctacac tcgcatttgg agtaaattga agaaccatgt cagtcctgga gctgcaaatg 841 accactacca tcagcgaagg caaaaaaCCA CCAAAATGCT GGTGTGTGTG GTGGTGGTGT 901 TTGCGGTCAG CTGGCTGCCT CTCCATGCCT TCCAGCTTGC CGTTGACATT GACAGCCAGG 961 TCCTGGACCT GAAGGAGTAC AAACTCATCT TCACAGTGTT CCACATTATC GCCATGTGCT 1021 CCACTTTTGC CAATCCCCTT CTCTATGGCT GGATGAACAG CAACTACAGA AAGGCTTTCC 1081 TCTCGGCCTT CCGCTGTGAG CAGCGGTTGG ATGCCATTCA CTCTGAGGTG TCCGTGACAT 1141 TCAAGGCTAA AAAGAACCTG GAGGTCAGAA AGAACAGTGG CCCCAATGAC TCTTTCACAG 1201 AGGCTACCAA TGTCTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACACTCG CATTCTAACT 1381 TGCCGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt