Construct: ORF TRCN0000491963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011095.2_s317c1
- Derived from:
- ccsbBroadEn_02739
- DNA Barcode:
- GCACTAACATACGTCAGGTTATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO28 (23219)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491963
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23219 | FBXO28 | F-box protein 28 | NM_015176.4 | 100% | 100% | |
| 2 | human | 23219 | FBXO28 | F-box protein 28 | NM_001136115.3 | 47.7% | 47% | (many diffs) |
| 3 | human | 23219 | FBXO28 | F-box protein 28 | NR_049764.2 | 17.7% | 1_19del;393_394ins139;985_5288del | |
| 4 | mouse | 67948 | Fbxo28 | F-box protein 28 | NM_175127.2 | 89.3% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1170
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagcggcg gaggagcgga tggcagagga aggaggcggc ggccaaggcg 121 acggcggttc ctctttggcc tccggctcta cccagcgaca gcctccaccg cccgcgccac 181 agcacccgca gccggggtcc caggcgctcc cagcccccgc gctggctccg gaccagctgc 241 ctcaaaacaa cacgcttgtg gcgctgccca tcgtagccat cgagaacatc ctcagcttta 301 tgtcctacga cgaaattagc cagctccgcc tggtttgtaa aagaatggac ttggtctgcc 361 agagaatgtt gaatcaggga tttctgaaag tggagaggta ccataatcta tgtcagaaac 421 aagttaaagc acaactccca aggagagagt cagaaaggag aaaccattca ttagctcgtc 481 atgcagacat tcttgctgct gttgaaacaa ggctgtcact attaaatatg actttcatga 541 aatatgtgga ttccaatctc tgttgcttca tcccaggaaa ggtgattgat gagatttatc 601 gtgtgttgag atatgtcaat tctaccagag cccctcaacg agctcatgaa gtacttcaag 661 aattaaggga tatatcctct atggcaatgg agtactttga tgaaaagatt gttccaattt 721 taaagaggaa attaccagga tcagatgttt ctggaagact catgggctct cctccagttc 781 caggaccgtc tgcagcccta acaacaatgc agctcttctc caagcaaaat ccttcaagac 841 aagaggttac caaactccag cagcaggtta aaacaaatgg tgctggcgtg actgttcTCA 901 GGCGTGAAAT TTCTGAGCTT CGCACCAAAG TGCAAGAACA GCAAAAACAG CTTCAAGACC 961 AGGACCAGAA ACTGCTAGAG CAGACCCAGA TCATAGGTGA ACAAAATGCA CGGTTGGCAG 1021 AGCTAGAACG CAAACTACGA GAAGTAATGG AAAGTGCTGT AGGAAATTCC TCAGGGTCCG 1081 GGCAGAATGA GGAGTCTCCT CGGAAACGAA AAAAGGCCAC GGAAGCCATA GACTCTCTTA 1141 GGAAATCTAA ACGTCTTCGG AATAGAAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1201 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1261 CGTAACTTGA AAGTATTTTG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCACT 1321 AACATACGTC AGGTTATACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1381 tgaaagatt