Construct: ORF TRCN0000491965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000301.1_s317c1
- Derived from:
- ccsbBroadEn_09397
- DNA Barcode:
- TAGGTGAGCCTATTTCAATACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYGB (114757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114757 | CYGB | cytoglobin | NM_134268.5 | 99.8% | 99.4% | 110A>G |
| 2 | human | 114757 | CYGB | cytoglobin | XM_005257005.3 | 90.4% | 88.7% | (many diffs) |
| 3 | human | 114757 | CYGB | cytoglobin | XM_017024116.1 | 58.9% | 57.3% | (many diffs) |
| 4 | mouse | 114886 | Cygb | cytoglobin | NM_030206.4 | 92.6% | 94.7% | (many diffs) |
| 5 | mouse | 114886 | Cygb | cytoglobin | XM_011248674.2 | 87.7% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaagtgcca ggcgagatgg agatcgagcg cagggagcgg agcgaggagc 121 tgtccgaggc ggagaggaag gcggtgcagg ctatgtgggc ccggctctat gccagctgcg 181 aggacgtggg ggtggccatc ctggtgaggt tctttgtgaa cttccccTCG GCCAAGCAGT 241 ACTTCAGCCA GTTCAAGCAC ATGGAGGATC CCCTGGAGAT GGAGCGGAGC CCCCAGCTGC 301 GGAAGCACGC CTGCCGAGTC ATGGGGGCCC TCAACACTGT CGTGGAGAAC CTGCATGACC 361 CCGACAAGGT GTCCTCTGTG CTCGCCCTTG TGGGGAAAGC CCACGCCCTC AAGCACAAGG 421 TGGAACCGGT GTACTTCAAG ATCCTCTCTG GGGTCATTCT GGAGGTGGTC GCCGAGGAAT 481 TTGCCAGTGA CTTCCCACCT GAGACGCAGA GAGCCTGGGC CAAGCTGCGT GGCCTCATCT 541 ACAGCCACGT GACCGCTGCC TACAAGGAAG TGGGCTGGGT GCAGCAGGTC CCCAACGCCA 601 CCACCCCACC GGCCACACTG CCCTCTTCGG GGCCGTACCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ATAGGTGAGC CTATTTCAAT ACAACACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt