Construct: ORF TRCN0000491973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019523.2_s317c1
- DNA Barcode:
- TTTCTTTGGCCCGAGTAGAGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGTR1 (185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491973
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 185 | AGTR1 | angiotensin II receptor type 1 | NM_000685.4 | 99.8% | 99.7% | 573C>T;1077_1078insG |
| 2 | human | 185 | AGTR1 | angiotensin II receptor type 1 | NM_009585.3 | 99.8% | 99.7% | 573C>T;1077_1078insG |
| 3 | human | 185 | AGTR1 | angiotensin II receptor type 1 | NM_032049.3 | 92.3% | 92.2% | 1_87del;660C>T;1164_1165insG |
| 4 | human | 185 | AGTR1 | angiotensin II receptor type 1 | NM_004835.4 | 90.9% | 90.8% | 1_105del;678C>T;1182_1183insG |
| 5 | human | 185 | AGTR1 | angiotensin II receptor type 1 | NM_031850.3 | 90.9% | 90.8% | 1_105del;678C>T;1182_1183insG |
| 6 | mouse | 11607 | Agtr1a | angiotensin II receptor, ty... | NM_177322.3 | 86.8% | 94.1% | (many diffs) |
| 7 | mouse | 11607 | Agtr1a | angiotensin II receptor, ty... | XM_006516534.1 | 86.8% | 94.1% | (many diffs) |
| 8 | mouse | 11607 | Agtr1a | angiotensin II receptor, ty... | XM_011244264.2 | 86.8% | 94.1% | (many diffs) |
| 9 | mouse | 11608 | Agtr1b | angiotensin II receptor, ty... | NM_175086.3 | 85.5% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1155
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgatt ctcaactctt ctactgaaga tggtattaaa agaatccaag 121 atgattgtcc caaagctgga aggcataatt acatatttgt catgattcct actttataca 181 gtatcatctt tgtggtggga atatttggaa acagcttggt ggtgatagtc atttactttt 241 atatgaagct gaagactgtg gccagtgttt ttcttttgaa tttagcactg gctgacttat 301 gctttttact gactttgcca ctatgggctg tctacacagc tatggaatac cgctggccct 361 ttggcaatta cctatgtaag attgcttcag ccagcgtcag tttcaacctg tacgctagtg 421 tgtttctact cacgtgtctc agcattgatc gatacctggc tattgttcac ccaatgaagt 481 cccgccttcg acgcacaatg cttgtagcca aagtcacctg catcatcatt tggctgctgg 541 caggcttggc cagtttgcca gctataatcc atcgaaatgt atttttcatt gagaacacca 601 atattacagt ttgtgctttc cattatgagt cccaaaattc aacccttccg atagggctgg 661 gcctgaccaa aaatatactg ggtttcctgt ttccttttct gatcattctt acaagttata 721 ctcttatttg gaaggcccta aagaaggctt atgaaattca gaagaacaaa cCAAGAAATG 781 ATGATATTTT TAAGATAATT ATGGCAATTG TGCTTTTCTT TTTCTTTTCC TGGATTCCCC 841 ACCAAATATT CACTTTTCTG GATGTATTGA TTCAACTAGG CATCATACGT GACTGTAGAA 901 TTGCAGATAT TGTGGACACG GCCATGCCTA TCACCATTTG TATAGCTTAT TTTAACAATT 961 GCCTGAATCC TCTTTTTTAT GGCTTTCTGG GGAAAAAATT TAAAAGATAT TTTCTCCAGC 1021 TTCTAAAATA TATTCCCCCA AAAGCCAAAT CCCACTCAAA CCTTTCAACA AAAATGAGCA 1081 CGCTTTCCTA CCGCCCCTCA GATAATGTAA GCTCATCCAC CAAGAAGCCT GCACCATGTT 1141 TTGAGGTTGA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT CTTTGGCCCG AGTAGAGATT 1321 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t