Construct: ORF TRCN0000491982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020867.2_s317c1
- DNA Barcode:
- AGATGAAATCGAAGTGTCCGTAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- P2RY12 (64805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_022788.4 | 99.9% | 100% | 36T>G |
| 2 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_176876.3 | 99.9% | 100% | 36T>G |
| 3 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | NM_027571.3 | 82.2% | 86.2% | (many diffs) |
| 4 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502083.3 | 82.2% | 86.2% | (many diffs) |
| 5 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502084.3 | 82.2% | 86.2% | (many diffs) |
| 6 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502085.3 | 82.2% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1098
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaagcc gtcgacaacc tcacctctgc gcctgggaac accagtctgt 121 gcaccagaga ctacaaaatc acccaggtcc tcttcccact gctctacact gtcctgtttt 181 ttgttggact tatcacaaat ggcctggcga tgaggatttt ctttcaaatc cggagtaaat 241 caaactttat tatttttctt aagaacacag tcatttctga tcttctcatg attctgactt 301 ttccattcaa aattcttagt gatgccaaac tgggaacagg accactgaga acttttgtgt 361 gtcaagttac ctccgtcata ttttatttca caatgtatat cagtatttca ttcctgggac 421 tgataactat cgatcgctac cagaagacca ccaggccatt taaaacatcc aaccccaaaa 481 atctcttggg ggctaagatt ctctctgttg tcatctgggc attcatgttc ttactctctt 541 tgcctaacat gattctgacc aacaggcagc cgagagacaa gaatgtgaag aaatgctctt 601 tccttaaatc agagttcggt ctagtctggc atgaaatagt aaattacatc tgtcaagtca 661 ttttctggat taatttcTTA ATTGTTATTG TATGTTATAC ACTCATTACA AAAGAACTGT 721 ACCGGTCATA CGTAAGAACG AGGGGTGTAG GTAAAGTCCC CAGGAAAAAG GTGAACGTCA 781 AAGTTTTCAT TATCATTGCT GTATTCTTTA TTTGTTTTGT TCCTTTCCAT TTTGCCCGAA 841 TTCCTTACAC CCTGAGCCAA ACCCGGGATG TCTTTGACTG CACTGCTGAA AATACTCTGT 901 TCTATGTGAA AGAGAGCACT CTGTGGTTAA CTTCCTTAAA TGCATGCCTG GATCCGTTCA 961 TCTATTTTTT CCTTTGCAAG TCCTTCAGAA ATTCCTTGAT AAGTATGCTG AAGTGCCCCA 1021 ATTCTGCAAC ATCTCTGTCC CAGGACAATA GGAAAAAAGA ACAGGATGGT GGTGACCCAA 1081 ATGAAGAGAC TCCAATGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAT GAAATCGAAG 1261 TGTCCGTAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt