Construct: ORF TRCN0000491984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020699.2_s317c1
- DNA Barcode:
- CTTCATGATGTGTCTAATTTGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK32B (55351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_018401.3 | 99.8% | 73.9% | 592A>G;955_956insT |
| 2 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_001345969.2 | 92.5% | 67.2% | 471_472ins90;502A>G;865_866insT |
| 3 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_001306082.2 | 88.4% | 63.4% | 0_1ins141;451A>G;814_815insT |
| 4 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_024454131.1 | 88.4% | 63.4% | 0_1ins141;451A>G;814_815insT |
| 5 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008374.1 | 54.2% | 51.8% | (many diffs) |
| 6 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_005247982.3 | 52.7% | 30.5% | 0_1ins585;7A>G;370_371insT |
| 7 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_005247983.4 | 52.7% | 30.5% | 0_1ins585;7A>G;370_371insT |
| 8 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_011513504.2 | 52.7% | 30.5% | 0_1ins585;7A>G;370_371insT |
| 9 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008377.1 | 52.7% | 30.5% | 0_1ins585;7A>G;370_371insT |
| 10 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008375.1 | 49.8% | 42.4% | (many diffs) |
| 11 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008376.1 | 49.8% | 42.4% | (many diffs) |
| 12 | mouse | 64293 | Stk32b | serine/threonine kinase 32B | NM_022416.2 | 86.6% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1317
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcggg aaccactccc acaagccccc cgtgtttgac gagaatgagg 121 aagtcaactt tgaccatttt cagattctgc gggccattgg taaagggagt tttggaaagg 181 tatgcatcgt gcagaagcga gacactaaga aaatgtatgc aatgaagtac atgaacaagc 241 agaagtgcat cgagagggat gaggttcgga atgttttccg ggagctgcag atcatgcaag 301 ggctggagca ccccttcctg gtcaatctgt ggtactcctt ccaggatgag gaggacatgt 361 tcatggtggt ggacctgctc ctgggaggcg acctgcgcta ccatctgcag cagaatgtgc 421 atttcacaga ggggactgtg aaactctaca tctgtgagct ggcactggcc ctggagtatc 481 ttcagaggta ccacatcatc cacagagaca tcaagccaga caatatcctg ctggatgaac 541 acggacatgt tcacattaca gacttcaaca tagcgacggt agtgaaagga gcagaaaggg 601 cttcctccat ggctggcacc aagccctaca tggctccaga agtattccag gtgtacatgg 661 acggaggccc cggatactcg taccctgtcg actggtggtc cctgggcatc acagcctatg 721 agctgctgcg gggctggagg ccgtacgaaa tccactcggt cacgcccatc gatgaaatcc 781 tcaacatgtt caaggtggag cgtgtccact actcctccac gtggtgcaag gggatggtgg 841 ccctgctgag gaagctcctg accaaggatc ctgagagccg cgtgtccagc cttcatgaca 901 tacagagcgt gccctacttg gccgacatga actgggacgc ggtgttcaag aaggcactga 961 tgcccggctt tgtgcccaat aaagggaggt tgaactgcga tcccacattt gagcttGAAG 1021 AGATGATTTC TAGAATCCAA GCCACTTCAC AAAAAGAAGA AGCGATTGGC AAAGAACAGA 1081 TCCAGGGATG GCACAAAGGA CAGCTGCCCG CTGAATGGAC ACCTGCAGCA CTGTTTGGAG 1141 ACTGTCCGGG AGGAATTCAT CATATTCAAC AGAGAGAAGC TCAGGAGGCA GCAGGGACAG 1201 GGCAGCCAGC TCTTGGACAC CGACAGCCGA GGGGGAGGCC AGGCCCAAAG CAAGCTCCAG 1261 GACGGGTGCA ACAACAACCT CCTCACCCAC ACCTGCACCC GTGGCTGCAG CAGCTAGAAC 1321 CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1381 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1441 TATATATCTT GTGGAAAGGA CGACTTCATG ATGTGTCTAA TTTGTTCACG CGTTAAGTCg 1501 acaatcaacc tctggattac aaaatttgtg aaagatt