Construct: ORF TRCN0000492008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021718.2_s317c1
- DNA Barcode:
- CTTATACTATTATCACAAAACACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHB1 (2047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2047 | EPHB1 | EPH receptor B1 | NM_004441.5 | 40% | 40% | 1_1770del |
| 2 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453389.1 | 38.7% | 38.7% | 1_1866del |
| 3 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453390.1 | 38.7% | 38.7% | 1_1866del |
| 4 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005867.1 | 38.1% | 38.1% | 1_1914del |
| 5 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005866.2 | 37.9% | 37.9% | 1_1932del |
| 6 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_001168296.1 | 37.4% | 36.9% | (many diffs) |
| 7 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_173447.3 | 36.8% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 114
- ORF end:
- 1296
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctacag cgataagctc cagcattaca gcacaggccg aggctcccca gggatgaaga 121 tctacattga ccccttcact tacgaggatc ccaacgaagc tgtccgggag tttgccaagg 181 agattgatgt atcttttgtg aaaattgaag aggtcatcgg agcaggggag tttggagaag 241 tgtacaaggg gcgtttgaaa ctgccaggca agagggaaat ctacgtggcc atcaagaccc 301 tgaaggcagg gtactcggag aagcagcgtc gggactttct gagtgaggcg agcatcatgg 361 gccagttcga ccatcctaac atcattcgcc tggagggtgt ggtcaccaag agtcggcctg 421 tcatgatcat cacagagttc atggagaatg gtgcattgga ttctttcctc aggcaaaatg 481 acgggcagtt caccgtgatc cagcttgtgg gtatgctcag gggcatcgct gctggcatga 541 agtacctggc tgagatgaat tatgtgcatc gggacctggc tgctaggaac attctggtca 601 acagtaacct ggtgtgcaag gtgtccgact ttggcctctc ccgctacctc caggatgaca 661 cctcagatcc cacctacacc agctccttgg gagggaagat ccctgtgaga tggacagctc 721 cagaggccat cgcctaccgc aagttcactt cagccagcga cgtttggagc tatgggatcg 781 tcatgtggga agtcatgtca tttggagaga gaccctattg ggatatgtcc aaccaagatg 841 tcatcaatgc catcgagcag gactaccggc tgcccccacc catggactgt ccagctgctc 901 tacaccagct catgctggac tgttggcaga aggaccggaa cagCCGGCCC CGGTTTGCGG 961 AGATTGTCAA CACCCTAGAT AAGATGATCC GGAACCCGGC AAGTCTCAAG ACTGTGGCAA 1021 CCATCACCGC CGTGCCTTCC CAGCCCCTGC TCGACCGCTC CATCCCAGAC TTCACGGCCT 1081 TTACCACCGT GGATGACTGG CTCAGCGCCA TCAAAATGGT CCAGTACAGG GACAGCTTCC 1141 TCACTGCTGG CTTCACCTCC CTCCAGCTGG TCACCCAGAT GACATCAGAA GACCTCCTGA 1201 GAATAGGCAT CACCTTGGCA GGCCATCAGA AGAAGATCCT GAACAGCATT CATTCTATGA 1261 GGGTCCAGAT AAGTCAGTCA CCAACGGCAA TGGCATGAGA CCCAGCTTTC TTGTACAAAG 1321 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1381 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1441 ACGACTTATA CTATTATCAC AAAACACAAC GCGTTAAGTC gacaatcaac ctctggatta 1501 caaaatttgt gaaagatt