Construct: ORF TRCN0000492011

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008974.1_s317c1
Derived from:
ccsbBroadEn_14271
DNA Barcode:
GCTAGCCTTTATCATAACACCTAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SUGCT (79783)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492011
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... NM_001193312.1 98.1% 47.3% (many diffs)
2 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515529.3 92.1% 44.4% (many diffs)
3 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... NM_001193313.1 88% 42.2% (many diffs)
4 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515527.3 86.8% 41.8% (many diffs)
5 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... NM_001193311.1 83.2% 39.9% (many diffs)
6 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_006715775.3 83.2% 39.9% (many diffs)
7 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515525.3 78.9% 37.8% (many diffs)
8 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515528.3 78% 44.1% (many diffs)
9 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_017012622.2 77% 43.2% (many diffs)
10 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... NM_024728.2 75.4% 32% (many diffs)
11 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515526.2 71% 30.1% (many diffs)
12 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_017012621.1 70.6% 29.5% (many diffs)
13 human 79783 SUGCT succinyl-CoA:glutarate-CoA ... XM_011515530.3 54% 63.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
639
ORF length:
570
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccatctgag acgcacgcga tgctggcgac gctggcgagg gtggcagctc
121tgcgcagaac ctgcctcttc tccggccggg gcggcgggag ggggctgtgg actggccgcc
181cgcagtcaga tatgaacaat ataaagccat tggaaggggt aaaaattctg gatctaacaa
241gagtcctggc gggacctttt gctactatga atttaggaga tcttggagca gaagttataa
301aagtggagag accaggagct ggtgatgata cacgaacttg ggggccacct tttgttggga
361cagaaagtac atattatctc agtgttaacc gaaataaaaa aagtattgct gttaatatca
421aggatccaaa aggggtgaaa atcatcaaag agcttgcagc tgtttgtgat gtgtttgtgg
481aaaactatgt ccctggcaaa ctgtctgcaa tgggcctggg atatgaagat atagacgaga
541ttgctcctca catcatctat tgttccatca cagggtatgg tcagacaggt ccaatttctc
601agcgagctgg ttatgatgct gttgcctcgg catgtttcta gntntgattc gcnnntcact
661ccaggatggc gtagctttgt ctcacgatga gctgcaagat tatcttattg gtcaaaagga
721agcaaaacgt tggggtacag ctcatggcag tatcgttcct taccaggctt ttaaaaccaa
781ggatggctat attgtagttg gagcaggaaa taaccagcag tttgccaccg tctgcaagat
841cttggatttg cctGAGTTGA TTGATAATTC CAAGTATAAA ACTAACCACC TTCGGGTACA
901CAATAGAAAA GAGCTTATTA AAATATTATC TGAACGGTTT GAAGAAGAAC TGACCAGCAA
961GTGGTTATAT CTTTTTGAAG GCAGTGGAGT CCCGTATGGC CCAATCAACA ACATGAAGAA
1021TGTATTTGCA GAACCTCAGG TATTACACAA TGGCCTCGTT ATGGAGATGG AGCATCCAAC
1081TGTGGGGAAG ATTTCCGTCC CAGGCCCAGC TGTGAGATAC AGTAAGTTCA AGATGTCAGA
1141GGCCAGGCCG CCCCCGCTGC TCGGGCAGCA CACAACGCAC ATCCTGAAGG AGGTCCTGAG
1201ATACGATGAC AGGGCCATCG GGGAGCTGCT CAGCGCTGGA GTGGTGGACC AACATGAAAC
1261TCACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT
1321CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC
1381TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCTAGCCTTT ATCATAACAC CTACACGCGT
1441TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)