Transcript: Human XM_017012621.1

PREDICTED: Homo sapiens succinyl-CoA:glutarate-CoA transferase (SUGCT), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SUGCT (79783)
Length:
1907
CDS:
253..1623

Additional Resources:

NCBI RefSeq record:
XM_017012621.1
NBCI Gene record:
SUGCT (79783)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012621.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153988 CCTTCGGGTACACAATAGAAA pLKO.1 1089 CDS 100% 5.625 7.875 N SUGCT n/a
2 TRCN0000153936 CTGGTGATGATACACGAACTT pLKO.1 380 CDS 100% 4.950 6.930 N SUGCT n/a
3 TRCN0000336747 CTGGTGATGATACACGAACTT pLKO_005 380 CDS 100% 4.950 6.930 N SUGCT n/a
4 TRCN0000158164 CGATCCGAATACACTGGCAAA pLKO.1 1653 3UTR 100% 4.050 5.670 N SUGCT n/a
5 TRCN0000350825 CGATCCGAATACACTGGCAAA pLKO_005 1653 3UTR 100% 4.050 5.670 N SUGCT n/a
6 TRCN0000152629 GTATTACACAATGGCCTCGTT pLKO.1 1396 CDS 100% 2.640 3.696 N SUGCT n/a
7 TRCN0000153099 GCAGGAAATAACCAGCAGTTT pLKO.1 1003 CDS 100% 4.950 3.960 N SUGCT n/a
8 TRCN0000336755 GCAGGAAATAACCAGCAGTTT pLKO_005 1003 CDS 100% 4.950 3.960 N SUGCT n/a
9 TRCN0000152533 GTATGGCCCAATCAACAACAT pLKO.1 1194 CDS 100% 4.950 3.960 N SUGCT n/a
10 TRCN0000336826 TCTCAGTGTTAACCGAAATAA pLKO_005 438 CDS 100% 15.000 10.500 N SUGCT n/a
11 TRCN0000158082 CCCAGCTGTGAGATACAGTAA pLKO.1 1461 CDS 100% 4.950 3.465 N SUGCT n/a
12 TRCN0000336825 CCCAGCTGTGAGATACAGTAA pLKO_005 1461 CDS 100% 4.950 3.465 N SUGCT n/a
13 TRCN0000153987 CCTGTATGCATATGGAGCTAT pLKO.1 777 CDS 100% 0.000 0.000 N SUGCT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012621.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08958 pDONR223 100% 86.3% 86.3% None (many diffs) n/a
2 ccsbBroad304_08958 pLX_304 0% 86.3% 86.3% V5 (many diffs) n/a
3 TRCN0000469305 GGGGCGAGCTCCCCTATAAGAGTT pLX_317 29.5% 86.3% 86.3% V5 (many diffs) n/a
4 ccsbBroadEn_14271 pDONR223 100% 70.6% 29.5% None (many diffs) n/a
5 ccsbBroad304_14271 pLX_304 0% 70.6% 29.5% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000492011 GCTAGCCTTTATCATAACACCTAC pLX_317 34.6% 70.6% 29.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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