Construct: ORF TRCN0000492014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014713.1_s317c1
- Derived from:
- ccsbBroadEn_16074
- DNA Barcode:
- TTCCAAACCCATTACCTTCAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOGA1 (112817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112817 | HOGA1 | 4-hydroxy-2-oxoglutarate al... | NM_001134670.1 | 100% | 100% | |
| 2 | human | 112817 | HOGA1 | 4-hydroxy-2-oxoglutarate al... | NM_138413.4 | 50.1% | 50.1% | 211_699del |
| 3 | mouse | 67432 | Hoga1 | 4-hydroxy-2-oxoglutarate al... | NM_026152.1 | 42% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggtccccaa gtctggtctt ctgtgaggca ggggctaagc aggagcttgt 121 ccaggaatgt gggggtctgg gcctcagggg aggggaagaa ggtggacatt gcgggtatct 181 acccccctgt gaccaccccc ttcactgcca ctgcagaggt ggacTATGGG AAACTGGAGG 241 AGAATCTGCA CAAACTGGGC ACCTTCCCCT TCCGAGGAGC TGTGGGGGGC GTCTGCGCCC 301 TGGCCAATGT CCTGGGGGCT CAGGTGTGCC AGCTGGAGCG ACTGTGCTGC ACGGGGCAAT 361 GGGAAGATGC CCAGAAACTG CAGCACCGCC TCATTGAGCC AAACGCTGCG GTGACCCGGC 421 GCTTTGGGAT CCCAGGGCTG AAGAAAATCA TGGACTGGTT TGGCTACTAT GGAGGCCCCT 481 GCCGCGCCCC CTTGCAGGAG CTGAGCCCCG CTGAGGAGGA GGCACTGCGC ATGGATTTCA 541 CCAGCAACGG CTGGCTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCCAAA CCCATTACCT 721 TCAACGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt