Construct: ORF TRCN0000492018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016805.1_s317c1
- Derived from:
- ccsbBroadEn_09767
- DNA Barcode:
- CCTCCCCTTCTTATTATAAGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIX1 (167410)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167410 | LIX1 | limb and CNS expressed 1 | NM_153234.5 | 99.8% | 99.6% | 8G>T |
| 2 | mouse | 66643 | Lix1 | limb and CNS expressed 1 | NM_025681.2 | 90% | 94.6% | (many diffs) |
| 3 | mouse | 66643 | Lix1 | limb and CNS expressed 1 | XM_006524797.3 | 46.3% | 41.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cataaccttg gaatctctga gacacatcat tgcccaagtc ttgcctcaca 121 gagatccggc tctagtcttc aaagacttga acgttgtgtc aatgttacag gaattttggg 181 aaagcaagca gcagcagaag gctgcattcc caagtgaagg tgtggtggtc tatgagtcac 241 tgccagctcc tgggcctccc tttgtgagtt acgtgaccct cccaggggga agctgttttg 301 gcaactttca gtgctgctta agtagagccg aggccaggcg ggatgcagct aaagtggccc 361 tgatcaactc cctcttcaat gagctgccct ctcgcaggat caccaaggaa ttcattatgg 421 aaagtgttca ggaagcagta gcctccacca gcggcacctt agatgatgca gatgacccca 481 gcaccagtgt tggggcctat cactacatgc tggagtcaaa catggggaag actatgctgg 541 agtttcagga gctgatgacc attttccaac tattgcactG GAATGGAAGC CTAAAAGCCC 601 TTCGTGAAAC AAAGTGTTCC CGACAGGAAG TCATCTCCTA CTATTCTCAG TATTCTCTAG 661 ATGAAAAGAT GCGCAGCCAC ATGGCCCTGG ACTGGATCAT GAAGGAGCGG GACTCACCAG 721 GAATTGTCTC TCAAGAGCTA CGAATGGCCC TGAGGCAGTT GGAGGAAGCC AGGAAAGCAG 781 GACAAGAACT ACGGTTTTAC AAAGAAAAGA AAGAAATATT GAGCTTAGCC CTGACTCAGA 841 TCTGCAGTGA CCCTGACACT TCCTCACCCA GTGATGATCA GCTGAGCCTT ACGGCCCTGT 901 GTGGCTATCA CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT CCCCTTCTTA TTATAAGGAA 1081 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t