Construct: ORF TRCN0000492022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014361.1_s317c1
- Derived from:
- ccsbBroadEn_07519
- DNA Barcode:
- TGCTGAGTCCCGGTAATACGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS3ST3A1 (9955)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9955 | HS3ST3A1 | heparan sulfate-glucosamine... | NM_006042.3 | 99.8% | 99.5% | 803G>A;891C>N |
| 2 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | NM_006041.3 | 77.2% | 67.8% | (many diffs) |
| 3 | human | 9955 | HS3ST3A1 | heparan sulfate-glucosamine... | XM_017025480.2 | 52.9% | 49.2% | (many diffs) |
| 4 | human | 9955 | HS3ST3A1 | heparan sulfate-glucosamine... | XM_011524114.3 | 50.6% | 50.2% | 1_2delATins599;206G>A;294C>N |
| 5 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | XM_017025479.2 | 49.7% | 49.5% | (many diffs) |
| 6 | human | 9953 | HS3ST3B1 | heparan sulfate-glucosamine... | NR_130138.2 | 34.6% | (many diffs) | |
| 7 | mouse | 15478 | Hs3st3a1 | heparan sulfate (glucosamin... | NM_178870.5 | 83.3% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccctccgggc ccggccagtg ccctctccac ctcggccgag ccgctgtccc 121 gcagcatctt ccggaagttc ttgctgatgc tctgctccct gctcacgtcc ctttacgtct 181 tctactgcct ggccgagcgc tgccagaccc tgtccggccc cgtcgtgggg ctgtccggcg 241 gcggcgagga ggcgggggcc cctggtggcg gcgtcctggc cggaggcccg agggagctgg 301 cggtgtggcc ggcggcggca cagagaaagc gcctcctgca actgccgcag tggcggaggc 361 gccggccgcc cgcgccccgc gacgacggcg aggaggcggc ctgggaagaa gagtcccctg 421 gcctgtcagg gggtccgggc ggctccgggg ccggaagcac cgtggccgag gccccgccgg 481 ggaccctggc gctgctcctg gacgaaggca gcaagcagct gccgcaggcc atcatcatcg 541 gagtgaagaa gggcggcacg cgggcgctgc tggagttcct gcgcgtgcac cccgacgtgc 601 gcgccgtggg cgccgagccc cacttcttcg accgcagcta cgacaagggc ctcgcctggt 661 accgggacct gatgcccaga accctggacg ggcagatcac catggagaag acgcccagtt 721 acttcgtcac gcgggaggcc cccgcgcgca tctcggccat gtccaaggac accaagctca 781 tcgtggtggt gcgggacccg gtgaccaggg ccatctcgga ctacacgcag acgctgtcca 841 agcggcccga catccccacc ttcgagaact tgacgttcaa aaacaggaca gcgggcctca 901 tcgacacgtc gtggagcgcc atccagatcg gcatctacgc caagcacctg gagcantggc 961 tgcgccactt ccccatccgc cagatgctct tcgtgagcgg cgagcggctc atcagcgacc 1021 cggccgggga gctgggccgc gtgcaagact tcctgggcct caagaggatc atcacggaca 1081 agcacttcta cTTCAACAAG ACCAAGGGCT TCCCCTGCCT GAAGAAGGCG GAGGGCAGCA 1141 GCCGGCCCCA TTGCCTGGGC AAGACCAAGG GCAGGACCCA TCCTGAGATC GACCGCGAGG 1201 TGGTGCGCAG GCTGCGCGAG TTCTACCGGC CTTTCAACCT CAAGTTCTAC CAGATGACCG 1261 GGCACGACTT TGGCTGGGAT GGATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GCTGAGTCCC 1441 GGTAATACGA CATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att