Transcript: Mouse NM_178870.5

Mus musculus heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 (Hs3st3a1), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Hs3st3a1 (15478)
Length:
3933
CDS:
736..1917

Additional Resources:

NCBI RefSeq record:
NM_178870.5
NBCI Gene record:
Hs3st3a1 (15478)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178870.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000098085 GCCTCATTACAAAGCTGTATT pLKO.1 2644 3UTR 100% 13.200 9.240 N Hs3st3a1 n/a
2 TRCN0000098086 CACTTCCCTCTACGTCTTCTA pLKO.1 834 CDS 100% 4.950 3.465 N Hs3st3a1 n/a
3 TRCN0000098087 CGACTTTGGCTGGGATGGATA pLKO.1 1896 CDS 100% 4.950 3.465 N Hs3st3a1 n/a
4 TRCN0000098088 CCTCGCCTGGTACCGGGATTT pLKO.1 1281 CDS 100% 0.000 0.000 N Hs3st3a1 n/a
5 TRCN0000098089 CCTGGCCTTACTTCTGGACGA pLKO.1 1116 CDS 100% 0.720 0.432 N Hs3st3a1 n/a
6 TRCN0000098347 CGAGAGCCTGACGTTCCGCAA pLKO.1 1494 CDS 100% 0.000 0.000 Y Hs3st3b1 n/a
7 TRCN0000445792 TTCTTGCTGATGCTCTGCTCC pLKO_005 808 CDS 100% 2.160 1.512 N HS3ST3A1 n/a
8 TRCN0000035817 CAAGCACTTCTACTTCAACAA pLKO.1 1710 CDS 100% 4.950 2.475 Y HS3ST3B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178870.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07519 pDONR223 100% 83.3% 83% None (many diffs) n/a
2 ccsbBroad304_07519 pLX_304 0% 83.3% 83% V5 (many diffs) n/a
3 TRCN0000492022 TGCTGAGTCCCGGTAATACGACAT pLX_317 16.3% 83.3% 83% V5 (many diffs) n/a
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