Construct: ORF TRCN0000492023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018495.1_s317c1
- Derived from:
- ccsbBroadEn_06574
- DNA Barcode:
- CGCAAGAGCACAAGGGTCTTCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MDH2 (4191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4191 | MDH2 | malate dehydrogenase 2 | NM_005918.4 | 99.9% | 99.7% | 26C>T |
| 2 | human | 4191 | MDH2 | malate dehydrogenase 2 | NM_001282403.2 | 87.4% | 87.2% | 26C>T;428_429ins126 |
| 3 | human | 4191 | MDH2 | malate dehydrogenase 2 | NM_001282404.2 | 68.3% | 68.3% | 0_1ins321 |
| 4 | mouse | 17448 | Mdh2 | malate dehydrogenase 2, NAD... | NM_008617.2 | 85.2% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1080
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ctccgccctc gcccggcctg tcagcgctgc tctccgccgc agcttcagca 121 cctcggccca gaacaatgct aaagtagctg tgctaggggc ctctggaggc atcgggcagc 181 cactttcact tctcctgaag aacagcccct tggtgagccg cctgaccctc tatgatatcg 241 cgcacacacc cggagtggcc gcagatctga gccacatcga gaccaaagcc gctgtgaaag 301 gctacctcgg acctgaacag ctgcctgact gcctgaaagg ttgtgatgtg gtagttattc 361 cggctggagt ccccagaaag ccaggcatga cccgggacga cctgttcaac accaatgcca 421 cgattgtggc caccctgacc gctgcctgtg cccagcactg cccggaagcc atgatctgcg 481 tcattgccaa tccggttaat tccaccatcc ccatcacagc agaagttttc aagaagcatg 541 gagtgtacaa ccccaacaaa atcttcggcg tgacgaccct ggacatcgtc agagccaaca 601 cctttgttgc agagctgaag ggtttggatc cagctcgagt caacgtccct gtcattggtg 661 gccatgctgg gaagaccatc atccccctga tctcTCAGTG CACCCCCAAG GTGGACTTTC 721 CCCAGGACCA GCTGACAGCA CTCACTGGGC GGATCCAGGA GGCCGGCACG GAGGTGGTCA 781 AGGCTAAAGC CGGAGCAGGC TCTGCCACCC TCTCCATGGC GTATGCCGGC GCCCGCTTTG 841 TCTTCTCCCT TGTGGATGCA ATGAATGGAA AGGAAGGTGT TGTGGAATGT TCCTTCGTTA 901 AGTCACAGGA AACGGAATGT ACCTACTTCT CCACACCGCT GCTGCTTGGG AAAAAGGGCA 961 TCGAGAAGAA CCTGGGCATC GGCAAAGTCT CCTCTTTTGA GGAGAAGATG ATCTCGGATG 1021 CCATCCCCGA GCTGAAGGCC TCCATCAAGA AGGGGGAAGA TTTCGTGAAG ACCCTGAAGT 1081 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGACGCAA GAGCACAAGG GTCTTCCGCA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt