Construct: ORF TRCN0000492032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007723.1_s317c1
- Derived from:
- ccsbBroadEn_06361
- DNA Barcode:
- TTGACTGCTTACAACATTAGGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFH (3075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3075 | CFH | complement factor H | NM_001014975.2 | 99.8% | 99.7% | 921A>C;1204C>T |
| 2 | human | 3075 | CFH | complement factor H | XM_017001108.2 | 99% | 98.8% | 921A>C;1204C>T;1337_1338delATinsGCTTTACCCTC |
| 3 | human | 3075 | CFH | complement factor H | NM_000186.4 | 36.2% | 36% | (many diffs) |
| 4 | human | 3075 | CFH | complement factor H | XR_001737134.2 | 32.8% | (many diffs) | |
| 5 | human | 10878 | CFHR3 | complement factor H related 3 | XR_241062.3 | 25.1% | (many diffs) | |
| 6 | human | 10878 | CFHR3 | complement factor H related 3 | NM_001166624.1 | 24.1% | 20.1% | (many diffs) |
| 7 | human | 10878 | CFHR3 | complement factor H related 3 | XR_426757.2 | 23.4% | (many diffs) | |
| 8 | human | 10878 | CFHR3 | complement factor H related 3 | NM_021023.5 | 21.7% | 18.1% | (many diffs) |
| 9 | human | 10878 | CFHR3 | complement factor H related 3 | XR_001736938.1 | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1413
- ORF length:
- 1347
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag acttctagca aagattattt gccttatgtt atgggctatt tgtgtagcag 121 aagattgcaa tgaacttcct ccaagaagaa atacagaaat tctgacaggt tcctggtctg 181 accaaacata tccagaaggc acccaggcta tctataaatg ccgccctgga tatagatctc 241 ttggaaatgt aataatggta tgcaggaagg gagaatgggt tgctcttaat ccattaagga 301 aatgtcagaa aaggccctgt ggacatcctg gagatactcc ttttggtact tttaccctta 361 caggaggaaa tgtgtttgaa tatggtgtaa aagctgtgta tacatgtaat gaggggtatc 421 aattgctagg tgagattaat taccgtgaat gtgacacaga tggatggacc aatgatattc 481 ctatatgtga agttgtgaag tgtttaccag tgacagcacc agagaatgga aaaattgtca 541 gtagtgcaat ggaaccagat cgggaatacc attttggaca agcagtacgg tttgtatgta 601 actcaggcta caagattgaa ggagatgaag aaatgcattg ttcagacgat ggtttttgga 661 gtaaagagaa accaaagtgt gtggaaattt catgcaaatc cccagatgtt ataaatggat 721 ctcctatatc tcagaagatt atttataagg agaatgaacg atttcaatat aaatgtaaca 781 tgggttatga atacagtgaa agaggagatg ctgtatgcac tgaatctgga tggcgtccgt 841 tgccttcatg tgaagaaaaa tcatgtgata atccttatat tccaaatggt gactactcac 901 ctttaaggat taaacacaga actggagatg aaatcacgta ccagtgtaga aatggttttt 961 atcctgcaac ccggggaaat acagccaaat gcacaagtac tggctggata cctgctccga 1021 gatgtaCCTT GAAACCTTGT GATTATCCAG ACATTAAACA TGGAGGTCTA TATCATGAGA 1081 ATATGCGTAG ACCATACTTT CCAGTAGCTG TAGGAAAATA TTACTCCTAT TACTGTGATG 1141 AACATTTTGA GACTCCGTCA GGAAGTTACT GGGATCACAT TCATTGCACA CAAGATGGAT 1201 GGTCGCCAGC AGTACCATGC CTCAGAAAAT GTTATTTTCC TTATTTGGAA AATGGATATA 1261 ATCAAAATTA TGGAAGAAAG TTTGTACAGG GTAAATCTAT AGACGTTGCC TGCCATCCTG 1321 GCTACGCTCT TCCAAAAGCG CAGACCACAG TTACATGTAT GGAGAATGGC TGGTCTCCTA 1381 CTCCCAGATG CATCCGTGTC AGCTTTACCC TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1441 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1501 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1561 GACTGCTTAC AACATTAGGG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1621 ttgtgaaaga tt