Construct: ORF TRCN0000492059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019193.2_s317c1
- DNA Barcode:
- ATATATCGGGACGATGGCGCCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LTB4R2 (56413)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56413 | LTB4R2 | leukotriene B4 receptor 2 | NM_001164692.2 | 92% | 92% | 0_1ins93 |
| 2 | human | 56413 | LTB4R2 | leukotriene B4 receptor 2 | NM_019839.5 | 92% | 92% | 0_1ins93 |
| 3 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519323.3 | 80.7% | 85.4% | (many diffs) |
| 4 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519325.3 | 80.7% | 85.4% | (many diffs) |
| 5 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_017316100.1 | 80.7% | 85.4% | (many diffs) |
| 6 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | NM_020490.2 | 78.4% | 84.4% | (many diffs) |
| 7 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519326.3 | 78.4% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1242
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggca ccttctcatc gggcatcaca ggtggggttt tgccccaccc 121 ctgaacgccc tctgtggcgc cttccaccca cctgtaggcc cagaaggatg tcggtctgct 181 accgtccccc agggaacgag acactgctga gctggaagac ttcgcgggcc acaggcacag 241 ccttcctgct gctggcggcg ctgctggggc tgcctggcaa cggcttcgtg gtgtggagct 301 tggcgggctg gcggcctgca cgggggcgac cgctggcggc cacgcttgtg ctgcacctgg 361 cgctggccga cggcgcggtg ctgctgctca cgccgctctt tgtggccttc ctgacccggc 421 aggcctggcc gctgggccag gcgggctgca aggcggtgta ctacgtgtgc gcgctcagca 481 tgtacgccag cgtgctgctc accggcctgc tcagcctgca gcgctgcctc gcagtcaccc 541 gccccttcct ggcgcctcgg ctgcgcagcc cggccctggc ccgccgcctg ctgctggcgg 601 tctggctggc cgccctgttg ctcgccgtcc cggccgccgt ctaccgccac ctgtggaggg 661 accgcgtatg ccagctgtgc cacccgtcgc cggtccacgc cgccgcccac ctgagcctgg 721 agactctgac cgctttcgtg cttcctttcg ggctgatgct cggctgctac agcgtgacgc 781 tggcacggct gcggggcgcc cgctggggct ccgggcggca cggggcgcgg gtgggccggc 841 tggtgagcgc catcgtgctt gccttcggct tgctctgggc cccctaccac gcagtcaacc 901 TTCTGCAGGC GGTCGCAGCG CTGGCTCCAC CGGAAGGGGC CTTGGCGAAG CTGGGCGGAG 961 CCGGCCAGGC GGCGCGAGCG GGAACTACGG CCTTGGCCTT CTTCAGTTCT AGCGTCAACC 1021 CGGTGCTCTA CGTCTTCACC GCTGGAGATC TGCTGCCCCG GGCAGGTCCC CGTTTCCTCA 1081 CGCGGCTCTT CGAAGGCTCT GGGGAGGCCC GAGGGGGCGG CCGCTCTAGG GAAGGGACCA 1141 TGGAGCTCCG AACTACCCCT CAGCTGAAAG TGGTGGGGCA GGGCCGCGGC AATGGAGACC 1201 CGGGGGGTGG GATGGAGAAG GACGGTCCGG AATGGGACCT TGACCCAGCT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAATA TATCGGGACG ATGGCGCCTA TACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t