Construct: ORF TRCN0000492066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004413.1_s317c1
- Derived from:
- ccsbBroadEn_05798
- DNA Barcode:
- AGGCCGAGACGGCTCGGATACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALAS1 (211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | NM_000688.6 | 99.8% | 99.8% | 519C>T;1904T>C |
| 2 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | NM_001304443.1 | 99.8% | 99.8% | 519C>T;1904T>C |
| 3 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | NM_199166.2 | 99.8% | 99.8% | 519C>T;1904T>C |
| 4 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | NM_001304444.1 | 97.3% | 97.2% | 1_51del;570C>T;1955T>C |
| 5 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | XM_017005873.1 | 91.3% | 91.2% | 519C>T;1163_1164ins165;1739T>C |
| 6 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | XM_024453393.1 | 91.3% | 91.2% | 519C>T;1163_1164ins165;1739T>C |
| 7 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | XM_011533477.2 | 89.3% | 88.9% | (many diffs) |
| 8 | human | 211 | ALAS1 | 5'-aminolevulinate synthase 1 | XM_011533478.2 | 88.9% | 88.8% | (many diffs) |
| 9 | mouse | 11655 | Alas1 | aminolevulinic acid synthase 1 | NM_001291835.1 | 87.1% | 91.4% | (many diffs) |
| 10 | mouse | 11655 | Alas1 | aminolevulinic acid synthase 1 | NM_020559.2 | 87.1% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtgttgtt cgccgctgcc cattcttatc ccgagtcccc caggcctttc 121 tgcagaaagc aggcaaatct ctgttgttct atgcccaaaa ctgccccaag atgatggaag 181 ttggggccaa gccagcccct cgggcattgt ccactgcagc agtacactac caacagatca 241 aagaaacccc tccggccagt gagaaagaca aaactgctaa ggccaaggtc caacagactc 301 ctgatggatc ccagcagagt ccagatggca cacagcttcc gtctggacac cccttgcctg 361 ccacaagcca gggcactgca agcaaatgcc ctttcctggc agcacagatg aatcagagag 421 gcagcagtgt cttctgcaaa gccagtcttg agcttcagga ggatgtgcag gaaatgaatg 481 ccgtgaggaa agaggttgct gaaacctcag caggccccag tgtggttagt gtgaaaaccg 541 atggagggga tcccagtgga ctgctgaaga acttccagga cattatgcaa aagcaaagac 601 cagaaagagt gtctcatctt cttcaagata acttgccaaa atctgtttcc acttttcagt 661 atgatcgttt ctttgagaaa aaaattgatg agaaaaagaa tgaccacacc tatcgagttt 721 ttaaaactgt gaaccggcga gcacacatct tccccatggc agatgactat tcagactccc 781 tcatcaccaa aaagcaagtg tcagtctggt gcagtaatga ctacctagga atgagtcgcc 841 acccacgggt gtgtggggca gttatggaca ctttgaaaca acatggtgct ggggcaggtg 901 gtactagaaa tatttctgga actagtaaat tccatgtgga cttagagcgg gagctggcag 961 acctccatgg gaaagatgcc gcactcttgt tttcctcgtg ctttgtggcc aatgactcaa 1021 ccctcttcac cctggctaag atgatgccag gctgtgagat ttactctgat tctgggaacc 1081 atgcctccat gatccaaggg attcgaaaca gccgagtgcc aaagtacatc ttccgccaca 1141 atgatgtcag ccacctcaga gaactgctgc aaagatctga cccctcagtc cccaagattg 1201 tggcatttga aactgtccat tcaatggatg gggcggtgtg cccactggaa gagctgtgtg 1261 atgtggccca tgagtttgga gcaatcacct tcgtggatga ggtccacgca gtggggcttt 1321 atggggctcg aggcggaggg attggggatc gggatggagt catgccaaaa atggacatca 1381 tttctggaac acttggcaaa gcctttggtt gtgttggagg gtacatcgcc agcacgagtt 1441 ctctgattga caccgtacgg tcctatgctg ctggcttcat cttcaccacc tctctgccac 1501 ccatgctgct ggctggagcc ctggagtctg tgcggatcct gaagagcgct gagggacggg 1561 tgcttcgccg ccagcaccag cgcaacgtca aactcatgag acagatgcta atggatgccg 1621 gcctccctgt tgtccactgc cccagccaca tcatccctgt gcgggttgca gatgcTGCTA 1681 AAAACACAGA AGTCTGTGAT GAACTAATGA GCAGACATAA CATCTACGTG CAAGCAATCA 1741 ATTACCCTAC GGTGCCCCGG GGAGAAGAGC TCCTACGGAT TGCCCCCACC CCTCACCACA 1801 CACCCCAGAT GATGAACTAC TTCCTTGAGA ATCTGCTAGT CACATGGAAG CAAGTGGGGC 1861 TGGAACTGAA GCCTCATTCC TCAGCTGAGT GCAACTTCTG CAGGAGGCCA CTGCATTTTG 1921 AAGTGATGAG TGAAAGAGAG AAGTCCTATT TCTCAGGCTT GAGCAAGTCG GTATCTGCTC 1981 AGGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGGCCGAGA CGGCTCGGAT ACTAAACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt