Construct: ORF TRCN0000492074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019231.2_s317c1
- DNA Barcode:
- TCAGCGTCTGACGCAACTTATGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRPR (2925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2925 | GRPR | gastrin releasing peptide r... | NM_005314.3 | 99.8% | 100% | 453T>C;663T>C |
| 2 | mouse | 14829 | Grpr | gastrin releasing peptide r... | NM_008177.3 | 87.5% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1227
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct ctaaatgact gtttccttct gaacttggag gtggaccatt 121 tcatgcactg caacatctcc agtcacagtg cggatctccc cgtgaacgat gactggtccc 181 acccggggat cctctatgtc atccctgcag tttatggggt tatcattctg ataggcctca 241 ttggcaacat cactttgatc aagatcttct gtacagtcaa gtccatgcga aacgttccaa 301 acctgttcat ttccagtctg gctttgggag acctgctcct cctaataacg tgtgctccag 361 tggatgccag caggtacctg gctgacagat ggctatttgg caggattggc tgcaaactga 421 tcccctttat acagcttacc tctgttgggg tgtctgtctt cacactcacg gcgctctcgg 481 cagacagata caaagccatt gtccggccaa tggatatcca ggcctcccat gccctgatga 541 agatctgcct caaagccgcc tttatctgga tcatctccat gctgctggcc attccagagg 601 ccgtgttttc tgacctccat cccttccatg aggaaagcac caaccagacc ttcattagct 661 gtgccccata cccacactct aatgagcttc accccaaaat ccattctatg gcttcctttc 721 tggtcttcta cgtcatccca ctgtcgatca tctctgttta ctactacttc attgctaaaa 781 atctgatcca gagtgcttac aaTCTTCCCG TGGAAGGGAA TATACATGTC AAGAAGCAGA 841 TTGAATCCCG GAAGCGACTT GCCAAGACAG TGCTGGTGTT TGTGGGCCTG TTCGCCTTCT 901 GCTGGCTCCC CAATCATGTC ATCTACCTGT ACCGCTCCTA CCACTACTCT GAGGTGGACA 961 CCTCCATGCT CCACTTTGTC ACCAGCATCT GTGCCCGCCT CCTGGCCTTC ACCAACTCCT 1021 GCGTGAACCC CTTTGCCCTC TACCTGCTGA GCAAGAGTTT CAGGAAACAG TTCAACACTC 1081 AGCTGCTCTG TTGCCAGCCT GGCCTGATCA TCCGGTCTCA CAGCACTGGA AGGAGTACAA 1141 CCTGCATGAC CTCCCTCAAG AGTACCAACC CCTCCGTGGC CACCTTTAGC CTCATCAATG 1201 GAAACATCTG TCACGAGCGG TATGTCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCAGCGTC 1381 TGACGCAACT TATGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt