Construct: ORF TRCN0000492085
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010016.1_s317c1
- Derived from:
- ccsbBroadEn_11625
- DNA Barcode:
- ATCACAATGGGTACAGTACTCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NID2 (22795)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492085
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22795 | NID2 | nidogen 2 | NM_007361.4 | 70.3% | 70.1% | (many diffs) |
| 2 | human | 22795 | NID2 | nidogen 2 | XM_005267405.5 | 68.9% | 68.8% | (many diffs) |
| 3 | human | 22795 | NID2 | nidogen 2 | XM_005267407.5 | 66.8% | 66.6% | (many diffs) |
| 4 | human | 22795 | NID2 | nidogen 2 | XM_005267406.5 | 65.5% | 65.3% | (many diffs) |
| 5 | mouse | 18074 | Nid2 | nidogen 2 | NM_008695.2 | 58.4% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2973
- ORF length:
- 2907
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggggaccgg gtggccgggc ggccggtgct gtcgtcgtta ccagtgctac 121 tgctgctgca gttgctaatg ttgcgggccg cggcgctgca cccagacgag ctcttcccac 181 acggggagtc gtggggggac cagctcctgc aggaaggcga cgacgaaagc tcagccgtgg 241 tgaagctggc gaatcccctg cacttctacg aagcccgatt cagcaacctc tacgtgggca 301 ccaacggcat catctccact caggacttcc ccagggaaac gcagtatgtg gactatgatt 361 tccccaccga cttcccggcc atcgcccctt ttctggcgga catcgacacg agccacggca 421 gaggccgagt cctgtaccga gtgaatggga aagtgagtgg ccacctccac gtgggccata 481 cacccgtgca cttcactgat gtggacctgc atgcgtatat cgtgggcaat gatggcagag 541 cctacacggc catcagccac atcccacagc cagcagccca ggccctcctc cccctcacac 601 caattggagg cctgtttggc tggctctttg ctttagaaaa acctggctct gagaacggct 661 tcagcctcgc aggtgctgcc tttacccatg acatggaagt tacattctac ccgggagagg 721 agacggttcg tatcactcaa actgctgagg gacttgaccc agagaactac ctgagcatta 781 agaccaacat tcaaggccag gtgccttacg tcccagcaaa tttcacagcc cacatctctc 841 cctacaagga gctgtaccac tactccgact ccactgtgac ctctacaagt tccagagact 901 actctctgac ttttggtgca atcaaccaaa catggtccta ccgcatccac cagaacatca 961 cttaccaggt gtgcaggcat gcccccagac acccgtcctt ccccaccacc cagcagctga 1021 acgtggaccg ggtctttgcc ttgtataatg acgaagaaag agtgcttaga tttgctgtga 1081 ccaatcaaat tggcccggtc aaagaagatt cagaccccac tccggtgaat ccttgctatg 1141 atgggagcca catgtgtgac acaacagcac ggtgccatcc agggacaggt gtagattaca 1201 cctgtgagtg cgcatctggg taccagggag atggacggaa ctgtgtggat gaaaatgaat 1261 gtgcaactgg ctttcatcgc tgtggcccca actctgtatg tatcaacttg cctggaagct 1321 acaggtgtga gtgccggagt gcttatgagt ttgcagatga ccggcatact tgcatcttga 1381 tcaccccacc tgccaacccc tgtgaggatg gcagtcatac ctgtgctcct gctgggcagg 1441 cccggtgtgt tcaccatgga ggcagcacgt tcagctgtgc ctgcctgcct ggttatgccg 1501 gcgatgggca ccagtgcact gatgtagatg aatgctcaga aaacagatgt caccctgcag 1561 ctacctgcta caatactcct ggttccttct cctgccgttg tcaacccgga tattatgggg 1621 atggatttca gtgcatacct gactccacct caagcctgac accctgtgaa caacagcagc 1681 gccatgccca ggcccagtat gcctaccctg gggcccggtt ccacatcccc caatgcgacg 1741 agcagggcaa cttcctgccc ctacagtgtc atggcagcac tggtttctgc tggtgcgtgg 1801 accctgatgg tcatgaagtt cctggtaccc agactccacc tggctccacc ccgcctcact 1861 gtggaccatc accagagccc acccagaggc ccccgaccat ctgtgagcgc tggagggaaa 1921 acctgctgga gcactacggt ggcacccccc gggatgacca gtacgtgccc cagtgcgatg 1981 acctgggcca cttcatcccc ctgcagtgcc acggaaagag cgacttctgc tggtgtgtgg 2041 acaaagatgg cagagaggtg cagggcaccc gctcccagcc aggcaccacc cctgcgtgta 2101 tacccaccgt cgctccaccc atggtccggc ccacgccccg gccagatgtg acccctccat 2161 ctgtgggcac cttcctgctc tatactcagg gccagcagat tggctactta cccctcaatg 2221 gcaccaggct tcagaaggat gcagctaaga ccctgctgtc tctgcatggc tccataatcg 2281 tgggaattga ttacgactgc cgggagagga tggtgtactg gacagatgtt gctggacgga 2341 caatcagccg tgccggtctg gaactgggag cagagcctga gacgatcgtg aattcaggtc 2401 tgataagccc tgaaggactt gccatagacc acatccgcag aacaatgtac tggacggaca 2461 gtgtcctgga taagatagag agcgccctgc tggatggctc tgagcgcaag gtcctcttct 2521 acacagatct ggtgaatCCC CGTGCCATCG CTGTGGATCC AATCCGAGGC AACTTGTACT 2581 GGACAGACTG GAATAGAGAA GCTCCTAAAA TTGAAACGTC ATCTTTAGAT GGAGAAAACA 2641 GAAGAATTCT GATCAATACA GACATTGGAT TGCCCAATGG CTTAACCTTT GACCCTTTCT 2701 CTAAACTGCT CTGCTGGGCA GATGCAGGAA CCAAAAAACT GGAGTGTACA CTACCTGATG 2761 GAACTGGACG GCGTGTCATT CAAAACAACC TCAAGTACCC CTTCAGCATC GTAAGCTATG 2821 CAGATCACTT CTACCACACA GACTGGAGGA GGGATGGTGT TGTATCAGTA AATAAACATA 2881 GTGGCCAGTT TACTGATGAG TATCTCCCAG AACAACGATC TCACCTCTAC GGGATAACTG 2941 CAGTCTACCC CTACTGCCCA ACAGGAAGAA AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 3001 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3061 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 3121 CACAATGGGT ACAGTACTCT GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 3181 ttgtgaaaga tt