Construct: ORF TRCN0000492089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021038.2_s317c1
- DNA Barcode:
- CCGACCACGACAGTCAATCGCGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAS1 (4142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492089
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4142 | MAS1 | MAS1 proto-oncogene, G prot... | NM_001366704.1 | 100% | 100% | |
| 2 | human | 4142 | MAS1 | MAS1 proto-oncogene, G prot... | NM_002377.4 | 100% | 100% | |
| 3 | mouse | 17171 | Mas1 | MAS1 oncogene | NM_008552.5 | 85.3% | 90.1% | (many diffs) |
| 4 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523293.4 | 85.3% | 90.1% | (many diffs) |
| 5 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523294.4 | 85.3% | 90.1% | (many diffs) |
| 6 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523296.4 | 85.3% | 90.1% | (many diffs) |
| 7 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246190.3 | 85.3% | 90.1% | (many diffs) |
| 8 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246191.3 | 85.3% | 90.1% | (many diffs) |
| 9 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246192.3 | 85.3% | 90.1% | (many diffs) |
| 10 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246193.3 | 85.3% | 90.1% | (many diffs) |
| 11 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_017317270.2 | 85.3% | 90.1% | (many diffs) |
| 12 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_030249523.1 | 85.3% | 90.1% | (many diffs) |
| 13 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_030249524.1 | 85.3% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1047
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatggg tcaaacgtga catcatttgt tgttgaggaa cccacgaaca 121 tctcaactgg caggaacgcc tcagtcggga atgcacatcg gcaaatcccc atcgtgcact 181 gggtcattat gagcatctcc ccagtggggt ttgttgagaa tgggattctc ctctggttcc 241 tgtgcttccg gatgagaaga aatcccttca ctgtctacat cacccacctg tctatcgcag 301 acatctcact gctcttctgt attttcatct tgtctatcga ctatgcttta gattatgagc 361 tttcttctgg ccattactac acaattgtca cattatcagt gacttttctg tttggctaca 421 acacgggcct ctatctgctg acggccatta gtgtggagag gtgcctgtca gtcctttacc 481 ccatctggta ccgatgccat cgccccaagt accagtcggc attggtctgt gcccttctgt 541 gggctctttc ttgcttggtg accaccatgg agtatgtcat gtgcatcgac agagaagaag 601 agagtcactc tcggaatgac tgccgagcag tcatcatctt tatagccatc cTGAGCTTCC 661 TGGTCTTCAC GCCCCTCATG CTGGTGTCCA GCACCATCTT GGTCGTGAAG ATCCGGAAGA 721 ACACGTGGGC TTCCCATTCC TCCAAGCTTT ACATAGTCAT CATGGTCACC ATCATTATAT 781 TCCTCATCTT CGCTATGCCC ATGAGACTCC TTTACCTGCT GTACTATGAG TATTGGTCGA 841 CCTTTGGGAA CCTACACCAC ATTTCCCTGC TCTTCTCCAC AATCAACAGT AGCGCCAACC 901 CTTTCATTTA CTTCTTTGTG GGAAGCAGTA AGAAGAAGAG ATTCAAGGAG TCCTTAAAAG 961 TTGTTCTGAC CAGGGCTTTC AAAGATGAAA TGCAACCTCG GCGCCAGAAA GACAATTGTA 1021 ATACGGTCAC AGTTGAGACT GTCGTCTAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGAC 1201 CACGACAGTC AATCGCGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt