Construct: ORF TRCN0000492092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020718.2_s317c1
- DNA Barcode:
- CCCCCGATGGCCCGCTTATCCTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BMP2K (55589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55589 | BMP2K | BMP2 inducible kinase | NM_017593.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_011532102.1 | 95% | 93.5% | (many diffs) |
| 3 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_005263117.1 | 58.1% | 57.9% | (many diffs) |
| 4 | human | 55589 | BMP2K | BMP2 inducible kinase | NM_198892.1 | 56.6% | 55.8% | (many diffs) |
| 5 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008381.1 | 49.7% | 48.9% | (many diffs) |
| 6 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008382.1 | 31.1% | 30.3% | (many diffs) |
| 7 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | XM_006534789.3 | 85.5% | 87.3% | (many diffs) |
| 8 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | XM_006534788.3 | 78.8% | 79.8% | (many diffs) |
| 9 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | NM_080708.1 | 49.8% | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2058
- ORF length:
- 1986
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagaag ttctctcgga tgcccaagtc ggagggcggc agcggcggcg 121 gagcggcggg tggcggggct ggcggggccg gggccggggc cggctgcggc tccggcggct 181 cgtccgtggg ggtccgggtg ttcgcggtcg gccgccacca ggtcaccctg gaagagtcgc 241 tggccgaagg tggattctcc acagttttcc tcgtgcgtac tcacggtgga atccgatgtg 301 cattgaagcg aatgtatgtc aataacatgc cagacctcaa tgtttgtaaa agggaaatta 361 caattatgaa agagctatct ggtcacaaaa atattgtggg ctatttggac tgtgctgtta 421 attcaattag tgataatgta tgggaagtcc ttatcttaat ggaatattgt cgagctggac 481 aggtagtgaa tcaaatgaat aagaagctac agacgggttt tacagaacca gaagtgttac 541 agatattctg tgatacctgt gaagctgttg caaggttgca tcagtgtaag actccaataa 601 ttcaccggga tctgaaggta gaaaatattt tgttgaatga tggtgggaac tatgtacttt 661 gtgactttgg cagtgccact aataaatttc ttaatcctca aaaagatgga gttaatgtag 721 tagaagaaga aattaaaaag tatacaactc tgtcatacag agcccctgaa atgatcaacc 781 tttatggagg gaaacccatc accaccaagg ctgatatctg ggcactggga tgtctactct 841 ataaactttg tttcttcact cttccttttg gtgagagtca ggttgctatc tgtgatggca 901 acttcaccat cccagacaat tctcgttact cccgtaacat acattgctta ataaggttca 961 tgcttgaacc agatccggaa catagacctg atatatttca agtgtcatat tttgcattta 1021 aatttgccaa aaaggattgt ccagtctcca acatcaataa ttcttctatt ccttcagctc 1081 ttcctgaacc gatggctgct agtgaagcag ctgctaggaa aagccaaata aaagccagaa 1141 taacagatac cattggacca acagaaacct caattgcacc aagacaaaga ccaaaggcca 1201 actctgctac tactgccact cccagtgtgc tgaccattca aagttcagca acacctgtta 1261 aagtccttgc tcctggtgaa ttcagtaacc atagaccaaa aggggcacta agacctggaa 1321 atggccctga aattttattg ggtcagggac ctcctcagca gccgccacag cagcatagag 1381 tactccagca actacagcag ggagattgga gattacagca actccattta cagcatcgtc 1441 atcctcacca gcagcagcag cagcagcagc agcaacagca acagcagcag cagcaacagc 1501 aacggcagca gcagcagcag cagcagcagc accaccacca ccaccaccac cacctacttc 1561 aagatgctta tatgcagcag tatcaacatg caacacagca gcaacagatg cttcaacaac 1621 aatttttaat gcattcggta tatcaaccac aaccttcTGC ATCACAGTAT CCTACAATGA 1681 TGCCGCAGTA TCAGCAGGCT TTCTTTCAAC AGCAGATGCT AGCTCAACAT CAGCCGTCTC 1741 AACAACAGGC ATCACCTGAA TATCTTACCT CCCCTCAAGA GTTCTCACCA GCCTTAGTTT 1801 CCTACACTTC ATCACTTCCA GCTCAGGTTG GAACCATAAT GGACTCCTCC TATAGTGCCA 1861 ATAGGTCAGT TGCTGATAAA GAGGCCATTG CAAATTTCAC AAATCAGAAG AACATCAGCA 1921 ATCCACCTGA TATGTCAGGG TGGAATCCTT TTGGAGAGGA TAATTTCTCT AAGTTAACAG 1981 AAGAGGAACT ATTGGACAGA GAATTTGACC TTCTAAGATC AAGTAAGGGA CACTTGAAGG 2041 CTTATTTTGC TTCACAATAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2101 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2161 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCCC CGATGGCCCG 2221 CTTATCCTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt