Construct: ORF TRCN0000492104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017117.4_s317c1
- Derived from:
- ccsbBroadEn_15051
- DNA Barcode:
- GTTATTGCCATCTCCAACGAGCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EEF2K (29904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492104
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29904 | EEF2K | eukaryotic elongation facto... | NM_013302.4 | 99.8% | 89.1% | 68A>G;489C>A;1930_1931insC |
| 2 | human | 29904 | EEF2K | eukaryotic elongation facto... | XM_017023193.2 | 99.8% | 88.9% | (many diffs) |
| 3 | human | 29904 | EEF2K | eukaryotic elongation facto... | XM_017023194.2 | 96.9% | 86% | (many diffs) |
| 4 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_950786.3 | 78.3% | (many diffs) | |
| 5 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_001751897.2 | 72.8% | (many diffs) | |
| 6 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_950787.3 | 51.6% | (many diffs) | |
| 7 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_001267710.1 | 85.7% | 80.5% | (many diffs) |
| 8 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_001267711.1 | 85.7% | 80.5% | (many diffs) |
| 9 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_007908.4 | 85.7% | 80.5% | (many diffs) |
| 10 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507342.3 | 85.7% | 80.5% | (many diffs) |
| 11 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507343.3 | 85.7% | 80.5% | (many diffs) |
| 12 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507344.3 | 80.4% | 75.1% | (many diffs) |
| 13 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507345.3 | 72.8% | 67% | (many diffs) |
| 14 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507346.3 | 69.5% | 64.1% | (many diffs) |
| 15 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XR_378214.2 | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2016
- ORF length:
- 1950
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agacgaagat ctcatcttcc gcctggaagg cgttgatggc ggccagtccc 121 cccgagctgg ccgtgatggt gattctgatg gggacagcga cgatgaggaa ggttacttca 181 tctgccccat cacggatgac ccaagctcga accagaatgt caattccaag gttaataagt 241 actacagcaa cctaacaaaa agtgagcggt atagctccag cgggtccccg gcaaactcct 301 tccacttcaa ggaagcctgg aagcacgcaa tccagaaggc caagcacatg cccgacccct 361 gggctgagtt ccacctggaa gatattgcca ccgaacgtgc tactcgacac aggtacaacg 421 ccgtcaccgg ggaatggctg gatgatgaag ttctgatcaa gatggcatct cagcccttcg 481 gccgaggagc aatgagggag tgcttccgga cgaagaagct ctccaacttc ttgcatgccc 541 agcagtggaa gggagcctcc aactacgtgg cgaagcgcta catcgagccc gtagaccggg 601 atgtgtactt tgaggacgtg cgtctacaga tggaggccaa gctctggggg gaggagtata 661 atcggcacaa gccccccaag caggtggaca tcatgcagat gtgcatcatc gagctgaagg 721 acagaccggg caagcccctc ttccacctgg agcactacat cgagggcaag tacatcaagt 781 acaactccaa ctctggcttt gtccgcgatg acaacatccg cctgacgccg caggccttca 841 gccacttcac ttttgagcgt tccggccatc agctgatagt ggtggacatc cagggagttg 901 gggatctcta cactgaccca cagatccaca cggagacggg cactgacttt ggagacggca 961 acctaggtgt ccgcgggatg gcgctcttct tctactctca tgcctgcaac cggatttgcg 1021 agagcatggg ccttgctccc tttgacctct cgccccggga gagggatgca gtgaatcaga 1081 acaccaagct gctgcaatca gccaagacca tcttgagagg aacagaggaa aaatgtggga 1141 gcccccgagt aaggaccctc tctgggagcc ggccacccct gctccgtccc ctttcagaga 1201 actctggaga cgagaacatg agcgacgtga ccttcgactc tctcccttct tccccatctt 1261 cggccacacc acacagccag aagctagacc acctccattg gccagtgttc agtgacctcg 1321 ataacatggc atccagagac catgatcatc tagacaacca ccgggagtct gagaatagtg 1381 gggacagcgg ataccccagt gagaagcggg gtgagctgga tgaccctgag ccccgagaac 1441 atggccactc atacagtaat cggaagtacg agtctgacga agacagcctg ggcagctctg 1501 gacgggtatg tgtagagaag tggaatctcc tcaactcctc ccgcctccac ctgccgaggg 1561 cttcggccgt ggccctggaa gtgcaaaggc ttaatgctct ggacctcgaa aagaaaatcg 1621 ggaagtccat tttggggaag gtccatctgg ccatggtgcg ctaccacgag ggtgggcgct 1681 tctgcgagaa gggcgaggag tgggaccagg agtcggctgt cttccacctg gagcacgcag 1741 ccaacctggg cgagctggag gccatcgtgg gcctgggact catgtactcg cagttgcctc 1801 atcacatcct agccgatgtc tctctgaagg agacagaaga gaacaaaacc aaaggatttg 1861 attacttact aaaggccgct gaagctggcg acaggcagtc catgatccta gtggcgcgag 1921 cttttgactc tggccagaac ctcagcccgg acaggtgcca agactggcta gaggccctgc 1981 actggtacaa cacTGCCCCT GGAGATGACG GACTGTGATG AGGGCGGTGA GTACGACGGA 2041 ATGCAGGACG AGCCCCGGTA CATGATGCTG GCCAGGGAGG CCGAGATGCT GTTCACAGGA 2101 GGCTACGGGC TGGAGAAGGA CCCGCAGAGA TCAGGGGACT TGTATACCCA GGCAGCAGAG 2161 GCAGCGATGG AAGCCATGAA GGGCCGACTG GCCAACCAGT ACTACCAAAA GGCTGAAGAG 2221 GCCTGGGCCC AGATGGAGGA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 2281 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 2341 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT ATTGCCATCT 2401 CCAACGAGCA AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 2461 t