Construct: ORF TRCN0000492110
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012515.2_s317c1
- Derived from:
- ccsbBroadEn_03008
- DNA Barcode:
- TTAATCGAGTGAAGGAAGAGGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNMT (27232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492110
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27232 | GNMT | glycine N-methyltransferase | NM_018960.6 | 100% | 100% | |
| 2 | human | 27232 | GNMT | glycine N-methyltransferase | NM_001318865.2 | 93.5% | 93.5% | 449_450ins57 |
| 3 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NM_001318856.2 | 76.7% | 76.6% | 1_1delAins203;4_7delCGAA |
| 4 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NM_001318857.2 | 71.8% | 61% | (many diffs) |
| 5 | human | 27232 | GNMT | glycine N-methyltransferase | NR_134899.2 | 68.9% | 1_14del;462_463ins143;757_933del | |
| 6 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NM_001318858.2 | 59.9% | 49.6% | (many diffs) |
| 7 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NR_134892.2 | 57.8% | (many diffs) | |
| 8 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NR_134890.2 | 52.9% | (many diffs) | |
| 9 | human | 107080644 | CNPY3-GNMT | CNPY3-GNMT readthrough | NR_134891.2 | 51.4% | (many diffs) | |
| 10 | mouse | 14711 | Gnmt | glycine N-methyltransferase | NM_010321.1 | 88.1% | 91.5% | (many diffs) |
| 11 | mouse | 14711 | Gnmt | glycine N-methyltransferase | XR_001782039.1 | 61.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggacagcgtg taccggaccc gctccctggg ggtggcggcc gaagggctcc 121 cggaccagta cgcggacggg gaggcggcgc gcgtgtggca gctgtatatc ggagacaccc 181 gcagccgcac cgccgagtac aaggcatggc tgcttgggct gctgcgccag cacggctgcc 241 agcgggtgct cgacgtagcc tgtggcactg gggtggactc cattatgctg gtggaagagg 301 gcttcagtgt gacgagtgtg gatgccagtg acaagatgct gaagtatgca cttaaggagc 361 gctggaaccg gcggcacgag cccgccttcg acaagtgggt catcgaagaa gccaactgga 421 tgactctgga caaagatgtg ccccagtcag cagagggtgg ctttgatgct gtcatctgcc 481 ttggaaacag tttcgctcac ttgcCAGACT GCAAAGGGGA CCAGAGTGAG CACCGGCTGG 541 CGCTGAAAAA CATTGCGAGC ATGGTGCGGG CAGGGGGCCT ACTGGTCATT GATCATCGCA 601 ACTACGACCA CATCCTCAGT ACAGGCTGTG CACCCCCAGG GAAGAACATC TACTATAAGA 661 GTGACTTGAC CAAGGACGTC ACAACATCAG TGCTGATAGT GAACAACAAG GCCCACATGG 721 TGACCCTGGA CTATACGGTG CAGGTGCCGG GGGCTGGCCA GGATGGCTCT CCTGGCTTGA 781 GTAAGTTCCG GCTCTCCTAC TACCCACACT GTCTGGCATC CTTCACGGAG CTGCTCCAAG 841 CAGCCTTCGG AGGTAAGTGC CAGCACAGCG TCCTGGGCGA CTTCAAGCCT TACAAGCCAG 901 GCCAAACCTA CATTCCCTGC TACTTCATCC ACGTGCTCAA GAGGACAGAC TGCCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGATTAA TCGAGTGAAG GAAGAGGATT ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt