Construct: ORF TRCN0000492112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007659.2_s317c1
- Derived from:
- ccsbBroadEn_03538
- DNA Barcode:
- ATGATTATGACTAATTATTGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DARS2 (55157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55157 | DARS2 | aspartyl-tRNA synthetase 2,... | NM_018122.5 | 100% | 100% | |
| 2 | human | 55157 | DARS2 | aspartyl-tRNA synthetase 2,... | NM_001365212.1 | 92% | 92% | 1191_1192ins153 |
| 3 | human | 55157 | DARS2 | aspartyl-tRNA synthetase 2,... | NM_001365213.2 | 72.9% | 70.3% | (many diffs) |
| 4 | mouse | 226539 | Dars2 | aspartyl-tRNA synthetase 2 ... | NM_172644.4 | 83.3% | 84.2% | (many diffs) |
| 5 | mouse | 226539 | Dars2 | aspartyl-tRNA synthetase 2 ... | XM_006496757.1 | 80.7% | 82.2% | (many diffs) |
| 6 | mouse | 226539 | Dars2 | aspartyl-tRNA synthetase 2 ... | XM_006496758.2 | 66% | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2001
- ORF length:
- 1935
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cttcccttct tggttaagtc agctgtacag gggtttatcc agacccatca 121 gaaggaccac ccaaccgatc tggggttctc tctacagaag tctgttgcag agttcacaga 181 ggagaattcc agaattcagt agctttgttg tccggaccaa cacatgtgga gagttgcgtt 241 cgtctcactt aggccaagaa gtcaccttgt gtggatggat tcagtaccga aggcaaaaca 301 cattcttggt cctaagagat ttcgatgggc ttgttcaagt tatcattccc caggatgagt 361 cggcagcctc tgtgaagaag attttatgtg aagcccctgt ggaatctgtg gtgcaagtgt 421 ctggtacagt catttcccgt cctgcaggac aagagaatcc aaaaatgcca acaggtgaga 481 ttgaaatcaa agttaaaaca gctgagcttc tgaatgcctg caagaagctg ccctttgaaa 541 ttaagaactt cgtgaagaaa acagaggctc ttcggttgca gtatcgctac ttagacttgc 601 gtagtttcca aatgcagtat aacctgcgac tgaggtccca gatggtcatg aaaatgcggg 661 aatatctctg taatctgcat gggtttgtgg atatagaaac ccccacattg tttaagagga 721 ccccaggggg tgccaaagag tttttagtac catccaggga acctggaaag ttttattctc 781 tccctcagag tcctcaacag tttaagcaac ttctgatggt tggcggttta gacagatatt 841 ttcaggttgc ccgatgttat cgagatgaag gttcaagacc agacagacag cctgagttta 901 ctcagattga catagagatg tcatttgtag accagactgg gatccagagt ttaattgagg 961 gtttgctcca gtattcctgg cccaatgaca aagatcctgt ggttgttcct tttcctacta 1021 tgacttttgc tgaggtgctg gccacctatg gaactgataa acctgacact cgctttggaa 1081 tgaagattat agatatcagt gatgtgttta gaaacacaga gattggattt cttcaagatg 1141 cacttagtaa gccccatgga actgtgaaag ccatatgtat ccctgaagga gcaaaatact 1201 taaaaaggaa agacattgaa tccattagaa actttgcagc tgaccatttt aatcaggaaa 1261 tcttacctgt attccttaac gccaatagaa actggaattc tccagttgct aatttcataa 1321 tggagtcaca aagactggaa ttaatcagac taatggagac ccaagaggaa gatgtggtcc 1381 tactaactgc tggagagcac aataaagcat gctctttgtt aggaaaatta cgactggaat 1441 gtgctgacct tctagaaaca agaggagtgg tgctccgtga ccccactctg ttctctttcc 1501 tttgggtggt agatttccca ctcttcctgc ccaaggagga aaatcccaga gagctGGAAT 1561 CGGCCCACCA CCCATTTACT GCTCCCCACC CCAGTGACAT ACATCTCCTG TACACTGAGC 1621 CCAAAAAGGC CCGTAGCCAA CACTATGACT TGGTTTTAAA TGGCAATGAA ATAGGAGGTG 1681 GTTCAATTCG AATTCACAAT GCAGAGCTGC AGCGTTATAT CCTGGCAACC TTACTAAAGG 1741 AGGATGTGAA AATGCTCTCC CATCTGCTCC AGGCTTTAGA TTATGGGGCA CCCCCTCATG 1801 GAGGAATTGC CTTAGGGTTA GACAGACTGA TATGCCTTGT CACTGGATCT CCAAGCATCA 1861 GAGATGTCAT AGCCTTCCCA AAGTCCTTCC GGGGACATGA CCTCATGAGC AATACCCCAG 1921 ATTCTGTCCC TCCTGAGGAA CTGAAGCCCT ATCATATCCG AGTCTCCAAG CCAACAGACT 1981 CCAAAGCAGA AAGAGCTCAT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2041 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2101 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATGA TTATGACTAA 2161 TTATTGATTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt