Transcript: Mouse NM_172644.4

Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Dars2 (226539)
Length:
3621
CDS:
545..2506

Additional Resources:

NCBI RefSeq record:
NM_172644.4
NBCI Gene record:
Dars2 (226539)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172644.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000102613 GCCTTACCATATCCACGTCTT pLKO.1 2422 CDS 100% 4.050 5.670 N Dars2 n/a
2 TRCN0000102611 CCCTTTGAGATTAAAGACTTT pLKO.1 1007 CDS 100% 4.950 3.465 N Dars2 n/a
3 TRCN0000102610 GCCAACACTATGACTTGGTTT pLKO.1 2112 CDS 100% 4.950 3.465 N Dars2 n/a
4 TRCN0000152874 GCCAACACTATGACTTGGTTT pLKO.1 2112 CDS 100% 4.950 3.465 N DARS2 n/a
5 TRCN0000281365 GCCAACACTATGACTTGGTTT pLKO_005 2112 CDS 100% 4.950 3.465 N DARS2 n/a
6 TRCN0000102612 CCTGGTGATAAAGACCCTCTA pLKO.1 1457 CDS 100% 4.050 2.835 N Dars2 n/a
7 TRCN0000102614 GTTACCGAGATGAAGGCTCAA pLKO.1 1332 CDS 100% 4.050 2.835 N Dars2 n/a
8 TRCN0000153000 GCCACCTATGGAACTGATAAA pLKO.1 1517 CDS 100% 13.200 9.240 N DARS2 n/a
9 TRCN0000281285 GCCACCTATGGAACTGATAAA pLKO_005 1517 CDS 100% 13.200 9.240 N DARS2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172644.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03538 pDONR223 100% 83.3% 84.2% None (many diffs) n/a
2 ccsbBroad304_03538 pLX_304 0% 83.3% 84.2% V5 (many diffs) n/a
3 TRCN0000492112 ATGATTATGACTAATTATTGATTT pLX_317 23.3% 83.3% 84.2% V5 (many diffs) n/a
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