Construct: ORF TRCN0000492120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019740.2_s317c1
- DNA Barcode:
- CCAATGTGTACTTTACACTCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM2 (1129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_000739.2 | 99.9% | 99.7% | 1398_1399insG |
| 2 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006626.2 | 99.9% | 99.7% | 1398_1399insG |
| 3 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006627.2 | 99.9% | 99.7% | 1398_1399insG |
| 4 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006628.2 | 99.9% | 99.7% | 1398_1399insG |
| 5 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006629.2 | 99.9% | 99.7% | 1398_1399insG |
| 6 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006630.1 | 99.9% | 99.7% | 1398_1399insG |
| 7 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006631.2 | 99.9% | 99.7% | 1398_1399insG |
| 8 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006632.2 | 99.9% | 99.7% | 1398_1399insG |
| 9 | human | 1129 | CHRM2 | cholinergic receptor muscar... | XM_024446648.1 | 99.9% | 99.7% | 1398_1399insG |
| 10 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | NM_203491.3 | 89.2% | 96.1% | (many diffs) |
| 11 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | XM_006506136.2 | 89.2% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 1488
- ORF length:
- 1401
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccaa 61 nnnnnnnnna aaaagcaggc ttcaccatga ataactcaac aaactcctct aacaatagcc 121 tggctcttac aagtccttat aagacatttg aagtggtgtt tattgtcctg gtggctggat 181 ccctcagttt ggtgaccatt atcgggaaca tcctagtcat ggtttccatt aaagtcaacc 241 gccacctcca gaccgtcaac aattactttt tattcagctt ggcctgtgct gaccttatca 301 taggtgtttt ctccatgaac ttgtacaccc tctacactgt gattggttac tggcctttgg 361 gacctgtggt gtgtgacctt tggctagccc tggactatgt ggtcagcaat gcctcagtta 421 tgaatctgct catcatcagc tttgacaggt acttctgtgt cacaaaacct ctgacctacc 481 cagtcaagcg gaccacaaaa atggcaggta tgatgattgc agctgcctgg gtcctctctt 541 tcatcctctg ggctccagcc attctcttct ggcagttcat tgtaggggtg agaactgtgg 601 aggatgggga gtgctacatt cagttttttt ccaatgctgc tgtcaccttt ggtacggcta 661 ttgcagcctt ctatttgcca gtgatcatca tgactgtgct atattggcac atatcccgag 721 ccagcaagag caggataaag aaggacaaga aggagcctgt tgccaaccaa gaccccgttt 781 ctccaagtct ggtacaagga aggatagtga agccaaacaa taacaacatg cccagcagtg 841 acgatggcct ggagcacaac aaaatccaga atggcaaagc ccccagggat cctgtgactg 901 aaaactgtgt tcagggagag gagaaggaga gctccaatga ctccacctca gtcagtgctg 961 ttgcctctaa tatgagagat gatgaaataa cccaggatga aaacacagtt tccacttccc 1021 tgggccattc caaagatgag aactctaagc aaacatgcat cagaattggc accaagaccc 1081 caaaaagtga ctcatgtacc ccaactaata ccaccgtGGA GGTAGTGGGG TCTTCAGGTC 1141 AGAATGGAGA TGAAAAGCAG AATATTGTAG CCCGCAAGAT TGTGAAGATG ACTAAGCAGC 1201 CTGCAAAAAA GAAGCCTCCT CCTTCCCGGG AAAAGAAAGT CACCAGGACA ATCTTGGCTA 1261 TTCTGTTGGC TTTCATCATC ACTTGGGCCC CATACAATGT CATGGTGCTC ATTAACACCT 1321 TTTGTGCACC TTGCATCCCC AACACTGTGT GGACAATTGG TTACTGGCTT TGTTACATCA 1381 ACAGCACTAT CAACCCTGCC TGCTATGCAC TTTGCAATGC CACCTTCAAG AAGACCTTTA 1441 AACACCTTCT CATGTGTCAT TATAAGAACA TAGGCGCTAC AAGGGACCCA GCTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA CCAATGTGTA CTTTACACTC ATTAACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt