Construct: ORF TRCN0000492127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003803.3_s317c1
- Derived from:
- ccsbBroadEn_07587
- DNA Barcode:
- CTCAAATGCTCCCGATAGATCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1A1 (10327)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | NM_001202413.1 | 99.8% | 100% | 558A>G |
| 2 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | NM_001202414.2 | 99.8% | 100% | 558A>G |
| 3 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | NM_006066.4 | 99.8% | 100% | 558A>G |
| 4 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | NM_153326.3 | 99.8% | 100% | 558A>G |
| 5 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | XM_011540491.2 | 65.7% | 65.8% | 0_1ins333;225A>G |
| 6 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | XM_011540492.2 | 65.7% | 65.8% | 0_1ins333;225A>G |
| 7 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | XR_001736908.2 | 57.9% | 1_267del;816_817ins200;1043_1138del | |
| 8 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | XR_002957622.1 | 57.9% | (many diffs) | |
| 9 | human | 10327 | AKR1A1 | aldo-keto reductase family ... | XR_001736909.1 | 49.8% | 1_484del;1033_1034ins200;1260_1355del | |
| 10 | mouse | 58810 | Akr1a1 | aldo-keto reductase family ... | NM_021473.3 | 87.6% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcttcctgt gttctactgc acactgggca gaagatgcct ctgattggtc 121 tgggtacctg gaagagtgag cctggtcagg taaaagcagc tgttaagtat gcccttagcg 181 taggctaccg ccacattgat tgtgctgcta tctacggcaa tgagcctgag attggggagg 241 ccctgaagga ggacgtggga ccaggcaagg cggtgcctcg ggaggagctg tttgtgacat 301 ccaagctgtg gaacaccaag caccaccccg aggatgtgga gcctgccctc cggaagactc 361 tggctgacct ccagctggag tatctggacc tgtacctgat gcactggcct tatgcctttg 421 agcggggaga caaccccttc cccaagaatg ctgatgggac tatatgctac gactccaccc 481 actacaagga gacttggaag gctctggagg cactggtggc taaggggctg gtgcaggcgc 541 tgggcctgtc caacttcaac agtcggcaga ttgatgacat actcagtgtg gcctccgtgc 601 gtccagctgt cttgcaggtg gagtgccacc catacttGGC TCAAAATGAG CTAATTGCCC 661 ACTGCCAAGC ACGTGGCCTG GAGGTAACTG CTTATAGCCC TTTGGGCTCC TCTGATCGTG 721 CATGGCGTGA TCCTGATGAG CCTGTCCTGC TGGAGGAACC AGTAGTCCTG GCATTGGCTG 781 AAAAGTATGG CCGATCTCCA GCTCAGATCT TGCTCAGGTG GCAGGTCCAG CGGAAAGTGA 841 TCTGCATCCC CAAAAGTATC ACTCCTTCTC GAATCCTTCA GAACATCAAG GTGTTTGACT 901 TCACCTTTAG CCCAGAAGAG ATGAAGCAGC TAAATGCCCT GAACAAAAAT TGGAGATATA 961 TTGTGCCTAT GCTTACGGTG GATGGGAAGA GAGTCCCAAG GGATGCAGGG CATCCTCTGT 1021 ACCCCTTTAA TGACCCGTAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCA AATGCTCCCG 1201 ATAGATCCCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt