Construct: ORF TRCN0000492127

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003803.3_s317c1
Derived from:
ccsbBroadEn_07587
DNA Barcode:
CTCAAATGCTCCCGATAGATCCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKR1A1 (10327)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492127
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10327 AKR1A1 aldo-keto reductase family ... NM_001202413.1 99.8% 100% 558A>G
2 human 10327 AKR1A1 aldo-keto reductase family ... NM_001202414.2 99.8% 100% 558A>G
3 human 10327 AKR1A1 aldo-keto reductase family ... NM_006066.4 99.8% 100% 558A>G
4 human 10327 AKR1A1 aldo-keto reductase family ... NM_153326.3 99.8% 100% 558A>G
5 human 10327 AKR1A1 aldo-keto reductase family ... XM_011540491.2 65.7% 65.8% 0_1ins333;225A>G
6 human 10327 AKR1A1 aldo-keto reductase family ... XM_011540492.2 65.7% 65.8% 0_1ins333;225A>G
7 human 10327 AKR1A1 aldo-keto reductase family ... XR_001736908.2 57.9% 1_267del;816_817ins200;1043_1138del
8 human 10327 AKR1A1 aldo-keto reductase family ... XR_002957622.1 57.9% (many diffs)
9 human 10327 AKR1A1 aldo-keto reductase family ... XR_001736909.1 49.8% 1_484del;1033_1034ins200;1260_1355del
10 mouse 58810 Akr1a1 aldo-keto reductase family ... NM_021473.3 87.6% 93.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1041
ORF length:
975
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcttcctgt gttctactgc acactgggca gaagatgcct ctgattggtc
121tgggtacctg gaagagtgag cctggtcagg taaaagcagc tgttaagtat gcccttagcg
181taggctaccg ccacattgat tgtgctgcta tctacggcaa tgagcctgag attggggagg
241ccctgaagga ggacgtggga ccaggcaagg cggtgcctcg ggaggagctg tttgtgacat
301ccaagctgtg gaacaccaag caccaccccg aggatgtgga gcctgccctc cggaagactc
361tggctgacct ccagctggag tatctggacc tgtacctgat gcactggcct tatgcctttg
421agcggggaga caaccccttc cccaagaatg ctgatgggac tatatgctac gactccaccc
481actacaagga gacttggaag gctctggagg cactggtggc taaggggctg gtgcaggcgc
541tgggcctgtc caacttcaac agtcggcaga ttgatgacat actcagtgtg gcctccgtgc
601gtccagctgt cttgcaggtg gagtgccacc catacttGGC TCAAAATGAG CTAATTGCCC
661ACTGCCAAGC ACGTGGCCTG GAGGTAACTG CTTATAGCCC TTTGGGCTCC TCTGATCGTG
721CATGGCGTGA TCCTGATGAG CCTGTCCTGC TGGAGGAACC AGTAGTCCTG GCATTGGCTG
781AAAAGTATGG CCGATCTCCA GCTCAGATCT TGCTCAGGTG GCAGGTCCAG CGGAAAGTGA
841TCTGCATCCC CAAAAGTATC ACTCCTTCTC GAATCCTTCA GAACATCAAG GTGTTTGACT
901TCACCTTTAG CCCAGAAGAG ATGAAGCAGC TAAATGCCCT GAACAAAAAT TGGAGATATA
961TTGTGCCTAT GCTTACGGTG GATGGGAAGA GAGTCCCAAG GGATGCAGGG CATCCTCTGT
1021ACCCCTTTAA TGACCCGTAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1081CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1141AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCA AATGCTCCCG
1201ATAGATCCCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)