Transcript: Human XR_001736908.2

PREDICTED: Homo sapiens aldo-keto reductase family 1 member A1 (AKR1A1), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AKR1A1 (10327)
Length:
1138
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001736908.2
NBCI Gene record:
AKR1A1 (10327)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001736908.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231970 ACTTCAACAGTCGGCAGATTG pLKO_005 755 3UTR 100% 10.800 15.120 N AKR1A1 n/a
2 TRCN0000046421 CCTTCAGAACATCAAGGTGTT pLKO.1 877 3UTR 100% 4.050 5.670 N AKR1A1 n/a
3 TRCN0000046420 GCAGCTGTTAAGTATGCCCTT pLKO.1 358 3UTR 100% 2.160 3.024 N AKR1A1 n/a
4 TRCN0000288810 GCAGCTGTTAAGTATGCCCTT pLKO_005 358 3UTR 100% 2.160 3.024 N AKR1A1 n/a
5 TRCN0000046418 GCAGCTAAATGCCCTGAACAA pLKO.1 928 3UTR 100% 4.950 3.960 N AKR1A1 n/a
6 TRCN0000231969 GAATGCTGATGGGACTATATG pLKO_005 648 3UTR 100% 13.200 9.240 N AKR1A1 n/a
7 TRCN0000231968 ACGGCAATGAGCCTGAGATTG pLKO_005 416 3UTR 100% 10.800 7.560 N AKR1A1 n/a
8 TRCN0000231967 AGGCTACCGCCACATTGATTG pLKO_005 384 3UTR 100% 10.800 7.560 N AKR1A1 n/a
9 TRCN0000046419 GCCACATTGATTGTGCTGCTA pLKO.1 392 3UTR 100% 2.640 1.848 N AKR1A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001736908.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07587 pDONR223 100% 57.9% None 1_267del;816_817ins200;1043_1138del n/a
2 ccsbBroad304_07587 pLX_304 0% 57.9% V5 1_267del;816_817ins200;1043_1138del n/a
3 TRCN0000492127 CTCAAATGCTCCCGATAGATCCCG pLX_317 41.6% 57.9% V5 1_267del;816_817ins200;1043_1138del n/a
4 ccsbBroadEn_02401 pDONR223 100% 57.9% None 1_267del;816_817ins200;1043_1138del n/a
5 ccsbBroad304_02401 pLX_304 0% 57.9% V5 1_267del;816_817ins200;1043_1138del n/a
6 TRCN0000472565 CCCTTCACCGTTGTTACACGGTTT pLX_317 43% 57.9% V5 1_267del;816_817ins200;1043_1138del n/a
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