Construct: ORF TRCN0000492128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015652.1_s317c1
- Derived from:
- ccsbBroadEn_12140
- DNA Barcode:
- GTGCGATGTCCGATACTACTGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP47 (55031)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55031 | USP47 | ubiquitin specific peptidas... | XM_011520196.1 | 16% | 16% | 1_2457del;2673G>A |
| 2 | human | 55031 | USP47 | ubiquitin specific peptidas... | XM_017017954.1 | 16% | 16% | 1_2457del;2673G>A |
| 3 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372102.1 | 13.2% | 13.2% | 1_3072del;3288G>A |
| 4 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372100.1 | 12.2% | 12.3% | 1_3354del;3570G>A |
| 5 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372099.1 | 12.1% | 12.2% | 1_3387del;3603G>A |
| 6 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_017944.4 | 12.1% | 12.1% | 1_3390del;3606G>A |
| 7 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372098.1 | 12% | 12% | 1_3444del;3660G>A |
| 8 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372097.1 | 11.9% | 11.9% | 1_3462del;3678G>A |
| 9 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372095.1 | 11.6% | 11.6% | 1_3573del;3789G>A |
| 10 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372093.1 | 11.6% | 11.6% | 1_3576del;3792G>A |
| 11 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372094.1 | 11.6% | 11.6% | 1_3576del;3792G>A |
| 12 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372092.1 | 11.5% | 11.6% | 1_3582del;3798G>A |
| 13 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372091.1 | 11.5% | 11.5% | 1_3591del;3807G>A |
| 14 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001282659.2 | 11.5% | 11.5% | 1_3594del;3810G>A |
| 15 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001330208.2 | 11.3% | 11.4% | 1_3654del;3870G>A |
| 16 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372103.1 | 11% | 11% | 1_3354del;3471_3472ins48;3522G>A |
| 17 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372101.1 | 7.6% | 7.4% | (many diffs) |
| 18 | human | 55031 | USP47 | ubiquitin specific peptidas... | NM_001372096.1 | 7.1% | 6.9% | (many diffs) |
| 19 | mouse | 74996 | Usp47 | ubiquitin specific peptidas... | NM_133758.3 | 10.4% | 10.7% | (many diffs) |
| 20 | mouse | 74996 | Usp47 | ubiquitin specific peptidas... | NM_177249.3 | 10.2% | 10.6% | (many diffs) |
| 21 | mouse | 74996 | Usp47 | ubiquitin specific peptidas... | XM_006508274.3 | 10.2% | 10.6% | (many diffs) |
| 22 | mouse | 74996 | Usp47 | ubiquitin specific peptidas... | XM_006508273.3 | 10.1% | 10.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtccatgtca cagcttgcag ttttgtcaag acggtggaag ccttcagaga 121 tgaagttGGA TCCCTTCCAG GAGGTTGTAT TGGAAAGCAG TAGTGTGGAC GAATTGCGAG 181 AGAAGCTTAG TGAAATCAGT GGGATTCCTT TGGATGATAT TGAATTTGCT AAGGGTAGAG 241 GAACATTTCC CTGTGATATT TCTGTCCTTG ATATTCATCA AGATTTAGAC TGGAATCCTA 301 AAGTTTCTAC CCTGAATGTC TGGCCTCTTT ATATCTGTGA TGATGGTGCG GTCATATTTT 361 ATAGGGATAA AACAGAAGAA TTAATGGAAT TGACAGATGA GCAAAGAAAT GAACTGATGA 421 AAAAAGAAAG CAGTCGACTC CAGAAGACTG GACATCGTGT AACATACTCA CCTCGTAAAG 481 AGAAAGCACT AAAAATATAT CTGGATGGAG CACCAAATAA AGATCTGACT CAAGACTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAGTGCGATG TCCGATACTA CTGTAGACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt