Construct: ORF TRCN0000492129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000259.1_s317c1
- Derived from:
- ccsbBroadEn_07699
- DNA Barcode:
- AGCTAGTGAAGCCGACCCCTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR83 (10888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10888 | GPR83 | G protein-coupled receptor 83 | NM_016540.4 | 99.9% | 100% | 1182T>C |
| 2 | human | 10888 | GPR83 | G protein-coupled receptor 83 | NM_001330345.2 | 89.9% | 90% | 386_387ins126;1056T>C |
| 3 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | NM_010287.3 | 85.4% | 88.9% | (many diffs) |
| 4 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | XM_006510017.2 | 81.6% | 84.9% | (many diffs) |
| 5 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | NM_001310734.1 | 76.8% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ccctcacctc ttgctgctct gtctcctccc cttggtgcga gccaccgagc 121 cccacgaggg ccgggccgac gagcagagcg cggaggcggc cctggccgtg cccaatgcct 181 cgcacttctt ctcttggaac aactacacct tctccgactg gcagaacttt gtgggcagga 241 ggcgctacgg cgctgagtcc cagaacccca cggtgaaagc cctgctcatt gtggcttact 301 ccttcatcat tgtcttctca ctctttggca acgtcctggt ctgtcatgtc atcttcaaga 361 accagcgaat gcactcggcc accagcctct tcatcgtcaa cctggcagtt gccgacataa 421 tgatcacgct gctcaacacc cccttcactt tggttcgctt tgtgaacagc acatggatat 481 ttgggaaggg catgtgccat gtcagccgct ttgcccagta ctgctcactg cacgtctcag 541 cactgacact gacagccatt gcggtggatc gccaccaggt catcatgcac cccttgaaac 601 cccggatctc aatcacaaag ggtgtcatct acatcgctgt catctggacc atggctacgt 661 tcttttcact cccacatgct atctgccaga aattatttac cttcaaatac agtgaggaca 721 ttgtgcgctc cctctgcctg ccagacttcc ctgagccagc tgacctcttc tggaagtacc 781 tggacttggc caccttcatc ctgctctaca tcctgcccct cctcatcatc tctgtggcct 841 acgctcgtgt ggccaagaaa ctgtggctgt gtaatatgat tggcgatgtg accacagagc 901 agtactttgc cctgcggcgc aaaaagaaga agaccatcaa gatgttgatg ctGGTGGTAG 961 TCCTCTTTGC CCTCTGCTGG TTCCCCCTCA ACTGCTACGT CCTCCTCCTG TCCAGCAAGG 1021 TCATCCGCAC CAACAATGCC CTCTACTTTG CCTTCCACTG GTTTGCCATG AGCAGCACCT 1081 GCTATAACCC CTTCATATAC TGCTGGCTGA ACGAGAACTT CAGGATTGAG CTAAAGGCAT 1141 TACTGAGCAT GTGTCAAAGA CCTCCCAAGC CTCAGGAGGA CAGGCCACCC TCCCCAGTTC 1201 CTTCCTTCAG GGTGGCCTGG ACAGAGAAGA ATGATGGCCA GAGGGCCCCC CTTGCCAATA 1261 ACCTCCTGCC CACCTCCCAA CTCCAGTCTG GGAAGACAGA CCTGTCATCT GTGGAACCCA 1321 TTGTGACGAT GAGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGCTAGTGAA GCCGACCCCT 1501 ACCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt