Construct: ORF TRCN0000492141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021522.2_s317c1
- DNA Barcode:
- TTCACCAAATGGCCTGTTCCTGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA1 (6195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_002953.4 | 99.9% | 100% | 51T>G;507T>C |
| 2 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001330441.2 | 96.2% | 95.9% | (many diffs) |
| 3 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001006665.2 | 95.8% | 95.3% | (many diffs) |
| 4 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | XM_024448871.1 | 87.4% | 87.4% | 0_1ins276;231T>C |
| 5 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_009097.5 | 91% | 98.5% | (many diffs) |
| 6 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285505.1 | 89.5% | 97% | (many diffs) |
| 7 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | XM_006538677.3 | 87.4% | 92.9% | (many diffs) |
| 8 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285506.1 | 86.8% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2274
- ORF length:
- 2205
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgctcgcc cagctcaagg agccctggcc gctcatggag ctagtgccgc 121 tggacccgga gaatggacag acctcagggg aagaagctgg acttcagccg tccaaggatg 181 agggcgtcct caaggagatc tccatcacgc accacgtcaa ggctggctct gagaaggctg 241 atccatccca tttcgagctc ctcaaggttc tgggccaggg atcctttggc aaagtcttcc 301 tggtgcggaa agtcacccgg cctgacagtg ggcacctgta tgctatgaag gtgctgaaga 361 aggcaacgct gaaagtacgt gaccgcgtcc ggaccaagat ggagagagac atcctggctg 421 atgtaaatca cccattcgtg gtgaagctgc actatgcctt ccagaccgag ggcaagctct 481 atctcattct ggacttcctg cgtggtgggg acctcttcac ccggctctca aaagaggtga 541 tgttcacgga ggaggatgtg aagttttacc tggccgagct ggctctgggc ctggatcacc 601 tgcacagcct gggtatcatt tacagagacc tcaagcctga gaacatcctt ctggatgagg 661 agggccacat caaactcact gactttggcc tgagcaaaga ggccattgac cacgagaaga 721 aggcctattc tttctgcggg acagtggagt acatggcccc tgaggtcgtc aaccgccagg 781 gccactccca tagtgcggac tggtggtcct atggggtgtt gatgtttgag atgctgacgg 841 gctccctgcc cttccagggg aaggaccgga aggagaccat gacactgatt ctgaaggcga 901 agctaggcat gccccagttt ctgagcactg aagcccagag cctcttgcgg gccctgttca 961 agcggaatcc tgccaaccgg ctcggctccg gccctgatgg ggcagaggaa atcaagcggc 1021 atgtcttcta ctccaccatt gactggaata agctataccg tcgtgagatc aagccaccct 1081 tcaagccagc agtggctcag cctgatgaca ccttctactt tgacaccgag ttcacgtccc 1141 gcacacccaa ggattcccca ggcatccccc ccagcgctgg ggcccatcag ctgttccggg 1201 gcttcagctt cgtggccacc ggcctgatgg aagacgacgg caagcctcgt gccccgcagg 1261 cacccctgca ctcggtggta cagcaactcc atgggaagaa cctggttttt agtgacggct 1321 acgtggtaaa ggagacaatt ggtgtgggct cctactctga gtgcaagcgc tgtgtccaca 1381 aggccaccaa catggagtat gctgtcaagg tcattgataa gagcaagcgg gatccttcag 1441 aagagattga gattcttctg cggtatggcc agcaccccaa catcatcact ctgaaagatg 1501 tgtatgatga tggcaaacac gtgtacctgg tgacagagct gatgcggggt ggggagctgc 1561 tggacaagat cctgcggcag aagttcttct cagagcggga ggccagcttt gtcctgcaca 1621 ccattggcaa aactgtggag tatctgcact cacagggggt tgtgcacagg gacctgaagc 1681 ccagcaacat cctgtatgtg gacgagtccg ggaatcccga gtgcctgcgc atctgtgact 1741 ttggttttgc caaacagctg cgggctgaga atgggctcct catgacacct tgctacacag 1801 ccaactttgt ggcgcctgag gtgctgaagc gccagggcta cgatgaaggc tgcgacatct 1861 ggagcctggg cattctgctg tacaccatgc tggcaggata tactccattt gccaacggtc 1921 ccagtgacac accagaggaa atcctaaccc ggatcggcag tgggaagttt acccTCAGTG 1981 GGGGAAATTG GAACACAGTT TCAGAGACAG CCAAGGACCT GGTGTCCAAG ATGCTACACG 2041 TGGATCCCCA CCAGCGCCTC ACAGCTAAGC AGGTTCTGCA GCATCCATGG GTCACCCAGA 2101 AAGACAAGCT TCCCCAAAGC CAGCTGTCCC ACCAGGACCT ACAGCTTGTG AAGGGAGCCA 2161 TGGCTGCCAC GTACTCCGCA CTCAACAGCT CCAAGCCCAC CCCCCAGCTG AAGCCCATCG 2221 AGTCATCCAT CCTGGCCCAG CGGCGAGTGA GGAAGTTGCC ATCCACCACC CTGTGAGACC 2281 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GATTCACCAA ATGGCCTGTT CCTGCAACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt