Construct: ORF TRCN0000492143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010200.1_s317c1
- Derived from:
- ccsbBroadEn_04179
- DNA Barcode:
- AACGGCCTTAATTATAAAACCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAA15 (80155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80155 | NAA15 | N(alpha)-acetyltransferase ... | NM_057175.5 | 100% | 100% | |
| 2 | human | 80155 | NAA15 | N(alpha)-acetyltransferase ... | XM_005263236.3 | 99.8% | 99.8% | 2154_2155insGCA |
| 3 | mouse | 74838 | Naa15 | N(alpha)-acetyltransferase ... | NM_053089.3 | 93% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2667
- ORF length:
- 2598
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggccgtg agcctcccgc ccaaggagaa tgcgctcttc aagcggatct 121 tgaggtgtta tgaacataaa cagtatagaa atggattgaa attctgtaaa caaatacttt 181 ctaatcccaa atttgcagag catggagaaa ccttggctat gaaaggatta acattgaact 241 gtttggggaa aaaggaagaa gcttatgaat tggttcgtag aggtttgaga aatgacttga 301 agagtcatgt gtgttggcac gtttatggcc ttcttcagag gtcagacaag aagtatgatg 361 aagccattaa gtgttacaga aatgcactaa aatgggataa agacaatctt caaatcttaa 421 gggacctttc cttactacag attcaaatgc gagatcttga gggttacagg gaaacgaggt 481 atcagttact tcagcttcga cctgcgcaga gagcatcatg gattggttat gctattgctt 541 accatttatt agaagattat gaaatggcag caaagatttt agaagaattt aggaaaacac 601 aacagacatc ccctgacaag gtggattatg aatatagtga actactctta tatcagaatc 661 aagttcttcg ggaagcaggt ctctatagag aagctttgga acatctttgt acctatgaaa 721 agcagatttg tgataaactt gctgtagaag aaaccaaagg ggaacttctg ttgcaactat 781 gtcgtttgga agatgctgca gatgtttata gaggattgca agagagaaat cctgaaaact 841 gggcctatta caaaggcttg gaaaaagcac tcaagccagc taatatgtta gaacggctaa 901 aaatttatga ggaagcctgg actaaatatc ccaggggact ggtgccaaga aggctgccgt 961 taaacttttt atctggtgag aagtttaaag aatgtttgga taagttccta aggatgaatt 1021 tcagcaaggg ttgcccacca gtcttcaata ctttaagatc attatacaaa gacaaagaaa 1081 aggtggcaat catagaagag ttagtagtag gttatgaaac ctctctaaaa agctgccggt 1141 tatttaaccc caatgatgat ggaaaggagg aaccaccaac cacattactt tgggtccagt 1201 actacttggc acaacattat gacaaaattg gtcagccatc tattgctttg gagtacataa 1261 atactgctat tgaaagtaca cctacattaa tagaactctt tctcgtgaaa gctaaaatct 1321 ataagcatgc tggaaatatt aaagaagctg caaggtggat ggatgaggcc caggccttgg 1381 acacagcaga cagatttatc aactccaaat gtgcaaaata catgctaaaa gccaacctga 1441 ttaaagaagc tgaagaaatg tgctcaaagt ttacaaggga aggaacatca gcggtagaga 1501 atttgaatga aatgcagtgc atgtggttcc aaacagaatg tgcccaggct tataaagcaa 1561 tgaataaatt tggtgaagca cttaagaaat gtcatgagat tgagagacat tttatagaaa 1621 tcactgatga ccagtttgac tttcatacat actgtatgag gaagattacc cttagatcat 1681 atgtggactt attaaaacta gaagatgtac ttcgacagca tccattttac ttcaaggcag 1741 caagaattgc tatagagatc tatttgaagc ttcatgacaa cccccttaca gatgagaata 1801 aagaacacga agctgataca gcaaacatgt ctgacaaaga gctaaagaag ctacgtaata 1861 aacaaagaag agctcaaaag aaagcccaga tagaagaaga gaaaaaaaat gcagaaaaag 1921 aaaagcagca gagaaatcag aaaaagaaga aggatgatga tgatgaggag ataggaggtc 1981 caaaagaaga acttattcca gagaaactgg ccaaggttga aactccattg gaagaagcta 2041 ttaaattttt aacaccgttg aagaacttgg tgaagaacaa gatagagact catctttttg 2101 cctttgagat ttactttagg aaagaaaagt ttcttttgat gctacaatca gtaaagaggg 2161 catttgctat tgattctagt catccctggc ttcatgagtg tatgattcgt ctctttaata 2221 ctgcagtgtg tgaaagtaaa gatttatctg atacagttag aacagtatta aaacaagaaa 2281 TGAATCGTCT TTTTGGAGCA ACGAATCCAA AGAATTTTAA TGAAACTTTT CTGAAAAGGA 2341 ATTCTGATTC ATTGCCACAC AGATTATCAG CTGCCAAAAT GGTATATTAC TTAGATCCTT 2401 CTAGTCAGAA GCGAGCTATA GAGTTGGCAA CAACACTTGA TGAATCTCTC ACTAACAGAA 2461 ACCTCCAGAC ATGTATGGAG GTATTGGAAG CCTTGTATGA TGGTAGCCTA GGAGACTGTA 2521 AAGAAGCTGC TGAAATTTAT AGAGCAAATT GTCATAAGCT TTTCCCTTAT GCTTTGGCTT 2581 TCATGCCTCC TGGATATGAA GAGGATATGA AGATCACAGT TAATGGAGAT AGTTCTGCAG 2641 AAGCTGAAGA ACTGGCCAAT GAAATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2701 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2761 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACGGCCT 2821 TAATTATAAA ACCACTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2881 aagatt