Construct: ORF TRCN0000492145

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009313.1_s317c1
Derived from:
ccsbBroadEn_04254
DNA Barcode:
GAATTCGACCTACGTCGTCCAAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VANGL1 (81839)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492145
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81839 VANGL1 VANGL planar cell polarity ... NM_001172412.1 100% 100%
2 human 81839 VANGL1 VANGL planar cell polarity ... NM_138959.3 100% 100%
3 human 81839 VANGL1 VANGL planar cell polarity ... NM_001172411.2 99.6% 99.6% 198_199insGTTCAG
4 mouse 229658 Vangl1 vang-like 1 (van gogh, Dros... NM_177545.5 88.4% 95% (many diffs)
5 mouse 229658 Vangl1 vang-like 1 (van gogh, Dros... XM_006501395.3 86.2% 92.7% (many diffs)
6 mouse 229658 Vangl1 vang-like 1 (van gogh, Dros... XM_017319549.1 76% 77.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1638
ORF length:
1572
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga taccgaatcc acttattctg gatattctta ctattcaagt cattcgaaaa
121aatctcacag acaaggggaa agaactagag agagacacaa gtcaccccgg aataaagacg
181gcagagggtc agaaaagtct gtcaccattc aacctcccac tggagagccc ctgttgggaa
241atgattctac tcggacagag gaagttcagg atgacaactg gggagagacc accacggcca
301tcacaggcac ctcggagcac agcatatccc aagaggacat tgccaggatc agcaaggaca
361tggaggacag cgtggggctg gattgcaaac gctacctggg cctcaccgtc gcctcttttc
421ttggacttct agttttcctc acccctattg ccttcatcct tttacctccg atcctgtgga
481gggatgagct ggagccttgt ggcacaattt gtgaggggct ctttatctcc atggcattca
541aactcctcat tctgctcata gggacctggg cacttttttt ccgcaagcgg agagctgaca
601tgccacgggt gtttgtgttt cgtgcccttt tgttggtcct catctttctc tttgtggttt
661cctattggct tttttacggg gtccgcattt tggactctcg ggaccggaat taccagggca
721ttgtgcaata tgcagtctcc cttgtggatg ccctcctctt catccattac ctggccatcg
781tcctgctgga gctcaggcag ctgcagccca tgttcacgct gcaggtggtc cgctccaccg
841atggcgagtc ccgcttctac agcctgggac acctgagtat ccagcgagca gcattggtgg
901tcctagaaaa ttactacaaa gatttcacca tctataaccc aaacctccta acagcctcca
961aattccgagc agccaagcat atggccgggc tgaaagtcta caatgtagat ggccccagta
1021acaatgccac tggccagtcc cgggccatga ttgctgcagc tgctcggcgc agggactcaa
1081gccacaacga gttgtattat gaagaggccg aacatgaacg gcgagtaaag aagcggaaag
1141caaggctggt ggttgcagtg gaagaggcct tcatccacat tcagcgtctc caggctgagg
1201agcagcagaa agccccaggg gaggtgatgg accctaggga ggccgcccag gccattttcc
1261cctccatggc cagggctctc cagaagtacc tgcgcatcac ccggcagcag aactaccaca
1321gcatggagag catcctgcag cacctggcct tctgcatcac caacggcatg acccccaagg
1381ccttcctaga acggtacctc agtgcgggcc ccaccctgca atatgacaag gaccgctggc
1441tctctacaca gtggaggctt gtcagtgatg aggctgtgac taatggatta cgggatggaa
1501ttgtgttcgt ccttaagtgc ttggacttca gcctcgtagt caatgtgaag aaaattccat
1561tcatcatact ctctgaagag ttcatagacc ccaaatctca caaatttgtc cttcgcttac
1621agtctGAGAC ATCCGTTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1681TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1741GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAATTCG ACCTACGTCG
1801TCCAAGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)