Construct: ORF TRCN0000492147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009347.2_s317c1
- Derived from:
- ccsbBroadEn_04118
- DNA Barcode:
- TCGCGTGTACACTTAGTATTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNIP1 (79753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79753 | SNIP1 | Smad nuclear interacting pr... | NM_024700.4 | 99.9% | 100% | 84G>A |
| 2 | mouse | 76793 | Snip1 | Smad nuclear interacting pr... | XM_011240648.1 | 62.9% | 66.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcggtgaag agcgaacggg agcgagggag ccggcgaaga caccgggacg 121 gggacgtggt gctgccggcg ggggtggtag tgaagcagga gcgtctcagc ccagaagtcg 181 cacctcccgc ccaccgccgt ccggaccact ccggtggtag cccgtctccg ccgaccagcg 241 agccggcccg ctcgggccac cgcgggaacc gagcccgagg agttagccgg tccccaccca 301 aaaagaaaaa caaggcctca gggagaagaa gcaagtctcc tcgcagtaag agaaaccgaa 361 gtcctcacca ctcaacagtc aaagtgaagc aggagcgtga ggatcatccc cggagaggac 421 gggaggatcg gcagcacagg gaaccatcag aacaggaaca caggagagct aggaacagtg 481 accgggacag acaccggggc cattcccacc aaaggagaac gtctaacgag aggcctggga 541 gtgggcaggg tcagggacgg gatcgagaca ctcagaacct gcaggctcag gaagaagagc 601 gggagtttta taatgccagg cgacgggagc atcgccagag gaatgacgtt ggtggtggcg 661 gcagtgagtc tcaggagttg gttcctcggc ctggtggcaa caacaaagaa aaagaggtgc 721 ccgctaaaga aaaaccaagc tttgaacttt ctggggcact tcttgaggac accaacactt 781 tccggggtgt agtcattaaa tatagtgagc ccccagaagc acgtatcccc aaaaaacggt 841 ggcgtctcta cccatttaaa aatgatgagg tgcttccagt catgtacata catcgacaga 901 gtgcgtaccT ACTGGGTCGA CACCGCCGCA TTGCAGACAT TCCAATTGAT CACCCGTCTT 961 GTTCAAAGCA GCATGCGGTC TTTCAATATC GGCTTGTGGA ATATACCCGT GCTGATGGCA 1021 CAGTTGGCCG AAGAGTGAAG CCCTACATCA TTGACCTTGG CTCAGGCAAT GGAACCTTCT 1081 TAAACAACAA ACGTATTGAG CCACAGAGAT ACTATGAACT AAAAGAAAAG GATGTACTCA 1141 AATTTGGATT CAGTAGCAGA GAATACGTCT TGCTCCATGA GTCGTCGGAC ACTTCTGAAA 1201 TAGACAGGAA AGATGACGAG GATGAGGAGG AGGAGGAAGA AGTGTCTGAC AGCTACCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAT CGCGTGTACA CTTAGTATTC AGCACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att