Construct: ORF TRCN0000492161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012438.2_s317c1
- Derived from:
- ccsbBroadEn_14232
- DNA Barcode:
- AGTGCTACATCAAGCCTTATCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1324 (57535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57535 | KIAA1324 | KIAA1324 | NM_001284353.1 | 97.7% | 97.7% | (many diffs) |
| 2 | human | 57535 | KIAA1324 | KIAA1324 | XM_017001883.2 | 94.1% | 94.1% | (many diffs) |
| 3 | human | 57535 | KIAA1324 | KIAA1324 | NM_001267048.2 | 71.2% | 71.2% | (many diffs) |
| 4 | human | 57535 | KIAA1324 | KIAA1324 | NM_001284352.2 | 70.6% | 70.4% | (many diffs) |
| 5 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541827.2 | 68.5% | 68.5% | (many diffs) |
| 6 | human | 57535 | KIAA1324 | KIAA1324 | NM_020775.5 | 65.1% | 65.1% | (many diffs) |
| 7 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541825.2 | 63.5% | 63.3% | (many diffs) |
| 8 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541826.3 | 63.5% | 63.2% | (many diffs) |
| 9 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | NM_001033304.1 | 63.5% | 65.3% | (many diffs) |
| 10 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501414.3 | 63.5% | 65.3% | (many diffs) |
| 11 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501415.3 | 63.5% | 65.3% | (many diffs) |
| 12 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_017319560.1 | 61.9% | 63.3% | (many diffs) |
| 13 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | NM_001346518.1 | 57.1% | 58.7% | (many diffs) |
| 14 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501412.2 | 55.7% | 56.9% | (many diffs) |
| 15 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501413.2 | 55.1% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caaatgggcc aagccgaaaa tctgtagcga ggaccttgag ggggcagtga 121 agctgcctgc ctctggtgtg aagacccact gcccaccctg caacccaggc ttcttcaaaa 181 ccaacaacag cacctgccag ccctgcccat atggttccta ctccaatggc tcagactgta 241 cccgctgccc tgcagggact gaacctgctg tgggatttga atacaaatgg tggaacacgc 301 tgcccacaaa catggaaacg accgttctca gtgggatcaa cttcgagtac aagggcatga 361 caggctggga ggtggctggt gatcacattt acacagctgc tggagcctca gacaatgact 421 tcatgattct cactctggtt gtgccaggat ttagacctcc gcagtcggtg atggcagaca 481 cagagaataa agaggtggcc agaatcacat ttgtctttga gaccctctgt tctgtgaact 541 gtgagctcta cttcatggtg ggtgtgaatt ctaggaccaa cactcctgtg gagacgtgga 601 aaggttccaa aggcaaacag tcctatacct acatcattga ggagaacact accacgagct 661 tcacctgggc cttccagagg accacttttc atgaggcaag caggaagtac accaatgacg 721 ttgccaagat ctactccatc aatgtcacca atgttatgaa tggcgtggcc tcctactgcc 781 gtccctgtgc cctagaagcc tctgatgtgg gctcctcctg cacctcttgt cctgctggtt 841 actatattga ccgagattca ggaacctgcc actcctgccc ccctaacaca attctgaaag 901 cccaccagcc ttatggtgtc caggcctgtg tgccctgtgg tccagggacc aagaacaaca 961 agatccactc tctgtgctac aatgattgca ccttctcacg caacactcca accaggactt 1021 tcaactacaa cttctccgct ttggcaaaca ccgtcactct tgctggaggg ccaagcttca 1081 cttccaaagg gttgaaatac ttccatcact ttaccctcag tctctgtgga aaccagggta 1141 ggaaaatgtc tgtgtgcacc gacaatgtca ctgacctccg gattcctgag ggtgagtcag 1201 ggttctccaa atctatcaca gcctacgtct gccaggcagt catcatcccc ccagaggtga 1261 caggctacaa ggccggggtt tcctcacagc ctgtcagcct tgctgatcga cttattgggg 1321 tgacaacaga tatgactctg gatggaatca cctccccagc tgaacttttc cacctggagt 1381 ccttgggaat accggacgtg atcttctttt ataggtccaa tgatgtgacc cagtcctgca 1441 gttctgggag atcaaccacc atccgcgtca ggtgcagtcc acagaaaact gtccctggaa 1501 gtttgctgct gccaggaacg tgctcagatg ggacctgtga tggctgcaac ttccacttcc 1561 tgtgggagag cgcggctgct tgcccgctct gctcagtggc tgactaccat gctatcgtca 1621 gcagctgtgt ggctgggatc cagaagacta cttacgtgtg gcgagaaccc aagctatgct 1681 ctggtggcat ttcTCTGCCT GAGCAGAGAG TCACCATCTG CAAAACCATA GATTTCTGGC 1741 TGAAAGTGGG CATCTCTGCA GGCACCTGTA CTGCCATCCT GCTCACCGTC TTGACCTGCT 1801 ACTTTTGGAA AAAGAATCAA AAACTAGAGT ACAAGTACTC CAAGCTGGTG ATGAATGCTA 1861 CTCTCAAGGA CTGTGACCTG CCAGCAGCTG ACAGCTGCGC CATCATGGAA GGCGAGGATG 1921 TAGAGGACGA CCTCATCTTT ACCAGCAAGA AGTCACTCTT TGGGAAGATC AAATCATTTA 1981 CCTCCAAGAG GACTCCTGAT GGATTTGACT CAGTGCCGCT GAAGACATCC TCAGGAGGCC 2041 CAGACATGGA GCTGTGCACA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2101 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2161 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGTGCTACAT CAAGCCTTAT 2221 CGATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt