Construct: ORF TRCN0000492162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015460.1_s317c1
- Derived from:
- ccsbBroadEn_01047
- DNA Barcode:
- GATGTCGTGGGCACATTGCCTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT1 (4521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367553.1 | 100% | 100% | |
| 2 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_002452.3 | 100% | 100% | |
| 3 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198948.1 | 100% | 100% | |
| 4 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198950.1 | 100% | 100% | |
| 5 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198953.1 | 100% | 100% | |
| 6 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198949.1 | 87.1% | 87.1% | 1_69del |
| 7 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198952.1 | 87.1% | 87.1% | 1_69del |
| 8 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198954.1 | 87.1% | 87.1% | 1_69del |
| 9 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367554.1 | 70.9% | 63.7% | (many diffs) |
| 10 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367555.1 | 70.9% | 63.7% | (many diffs) |
| 11 | human | 4521 | NUDT1 | nudix hydrolase 1 | NR_160264.1 | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 537
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgcctcc aggctctata ccctggtgct ggtcctgcag cctcagcgag 121 ttctcctggg catgaaaaag cgaggcttcg gggccggccg gtggaatggc tttGGGGGCA 181 AAGTGCAAGA AGGAGAGACC ATCGAGGATG GGGCTAGGAG GGAGCTGCAG GAGGAGAGCG 241 GTCTGACAGT GGACGCCCTG CACAAGGTGG GCCAGATCGT GTTTGAGTTC GTGGGCGAGC 301 CTGAGCTCAT GGACGTGCAT GTCTTCTGCA CAGACAGCAT CCAGGGGACC CCCGTGGAGA 361 GCGACGAAAT GCGCCCATGC TGGTTCCAGC TGGATCAGAT CCCCTTCAAG GACATGTGGC 421 CCGACGACAG CTACTGGTTT CCACTCCTGC TTCAGAAGAA GAAATTCCAC GGGTACTTCA 481 AGTTCCAGGG TCAGGACACC ATCCTGGACT ACACACTCCG CGAGGTGGAC ACGGTCTTGC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAGATGTCGT GGGCACATTG CCTGAGACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt