Construct: ORF TRCN0000492171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021014.2_s317c1
- DNA Barcode:
- GCCTTCATTTAGCGTCAGACGATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRM2 (1129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_000739.2 | 100% | 100% | |
| 2 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006626.2 | 100% | 100% | |
| 3 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006627.2 | 100% | 100% | |
| 4 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006628.2 | 100% | 100% | |
| 5 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006629.2 | 100% | 100% | |
| 6 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006630.1 | 100% | 100% | |
| 7 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006631.2 | 100% | 100% | |
| 8 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006632.2 | 100% | 100% | |
| 9 | human | 1129 | CHRM2 | cholinergic receptor muscar... | XM_024446648.1 | 100% | 100% | |
| 10 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | NM_203491.3 | 89.3% | 96.3% | (many diffs) |
| 11 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | XM_006506136.2 | 89.3% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1470
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaataac tcaacaaact cctctaacaa tagcctggct cttacaagtc 121 cttataagac atttgaagtg gtgtttattg tcctggtggc tggatccctc agtttggtga 181 ccattatcgg gaacatccta gtcatggttt ccattaaagt caaccgccac ctccagaccg 241 tcaacaatta ctttttattc agcttggcct gtgctgacct tatcataggt gttttctcca 301 tgaacttgta caccctctac actgtgattg gttactggcc tttgggacct gtggtgtgtg 361 acctttggct agccctggac tatgtggtca gcaatgcctc agttatgaat ctgctcatca 421 tcagctttga caggtacttc tgtgtcacaa aacctctgac ctacccagtc aagcggacca 481 caaaaatggc aggtatgatg attgcagctg cctgggtcct ctctttcatc ctctgggctc 541 cagccattct cttctggcag ttcattgtag gggtgagaac tgtggaggat ggggagtgct 601 acattcagtt tttttccaat gctgctgtca cctttggtac ggctattgca gccttctatt 661 tgccagtgat catcatgact gtgctatatt ggcacatatc ccgagccagc aagagcagga 721 taaagaagga caagaaggag cctgttgcca accaagaccc cgtttctcca agtctggtac 781 aaggaaggat agtgaagcca aacaataaca acatgcccag cagtgacgat ggcctggagc 841 acaacaaaat ccagaatggc aaagccccca gggatcctgt gactgaaaac tgtgttcagg 901 gagaggagaa ggagagctcc aatgactcca cctcagtcag tgctgttgcc tctaatatga 961 gagatgatga aataacccag gatgaaaaca cagtttccac ttccctgggc cattccaaag 1021 atgagaactc taagcaaaca tgcatcagaa ttGGCACCAA GACCCCAAAA AGTGACTCAT 1081 GTACCCCAAC TAATACCACC GTGGAGGTAG TGGGGTCTTC AGGTCAGAAT GGAGATGAAA 1141 AGCAGAATAT TGTAGCCCGC AAGATTGTGA AGATGACTAA GCAGCCTGCA AAAAAGAAGC 1201 CTCCTCCTTC CCGGGAAAAG AAAGTCACCA GGACAATCTT GGCTATTCTG TTGGCTTTCA 1261 TCATCACTTG GGCCCCATAC AATGTCATGG TGCTCATTAA CACCTTTTGT GCACCTTGCA 1321 TCCCCAACAC TGTGTGGACA ATTGGTTACT GGCTTTGTTA CATCAACAGC ACTATCAACC 1381 CTGCCTGCTA TGCACTTTGC AATGCCACCT TCAAGAAGAC CTTTAAACAC CTTCTCATGT 1441 GTCATTATAA GAACATAGGC GCTACAAGGT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1621 CTTCATTTAG CGTCAGACGA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt