Construct: ORF TRCN0000492180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021273.2_s317c1
- DNA Barcode:
- GAGCGTTCAAATGCTATAAACGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RARG (5916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5916 | RARG | retinoic acid receptor gamma | NM_000966.6 | 99.9% | 100% | 849G>A |
| 2 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449113.1 | 99.9% | 100% | 849G>A |
| 3 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001042728.3 | 91.1% | 88.7% | (many diffs) |
| 4 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243730.1 | 84% | 84.1% | 0_1ins216;633G>A |
| 5 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449114.1 | 84% | 84.1% | 0_1ins216;633G>A |
| 6 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449112.1 | 83.8% | 83.9% | 1_261del;1110G>A |
| 7 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243732.2 | 82.6% | 77.8% | (many diffs) |
| 8 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243731.2 | 73.2% | 73.3% | 0_1ins363;486G>A |
| 9 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | NM_011244.4 | 88.8% | 97.1% | (many diffs) |
| 10 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | XM_006520650.1 | 88.8% | 97.1% | (many diffs) |
| 11 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | XM_011245525.2 | 88.8% | 97.1% | (many diffs) |
| 12 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | NM_001042727.2 | 81.8% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1434
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccacc aataaggagc gactctttgc ggctggtgcc ctggggcctg 121 gatctggcta cccaggggca ggtttcccct tcgccttccc aggggcactc agggggtctc 181 cgcctttcga gatgctgagc cctagcttcc ggggcctggg ccagcctgac ctccccaagg 241 agatggcctc tctgtcggtg gagacacaga gcaccagctc agaggagatg gtgcccagct 301 cgccctcgcc ccctccgcct cctcgggtct acaagccatg cttcgtgtgc aatgacaagt 361 cctctggcta ccactatggg gtcagctctt gtgaaggctg caagggcttc tttcgccgaa 421 gcatccagaa gaacatggtg tacacgtgtc accgcgacaa aaactgtatc atcaacaagg 481 tgaccaggaa tcgctgccag tactgccggc tacagaagtg cttcgaagtg ggcatgtcca 541 aggaagctgt gcgaaatgac cggaacaaga agaagaaaga ggtgaaggaa gaagggtcac 601 ctgacagcta tgagctgagc cctcagttag aagagctcat caccaaggtc agcaaagccc 661 atcaggagac tttcccctcg ctctgccagc tgggcaagta taccacgaac tccagtgcag 721 accaccgcgt gcagctggat ctggggctgt gggacaagtt cagtgagctg gctaccaagt 781 gcatcatcaa gatcgtggag tttgccaagc ggttgcctgg ctttacaggg ctcagcattg 841 ctgaccagat cactctgctc aaagctgcct gcctagatat cctgatgctg cgtatctgca 901 caaggtacac cccagagcaa gacaccatga ccttctccga cgggctgacc ctgaaccgga 961 cccagatgca caatgccggc ttcgggcccc tcacagacct tgtctttgcc tttgctgggc 1021 agctcctgcc cctggagatg gatgacaccg agacagggct gctcagcgcc atctgcctca 1081 TCTGCGGAGA CCGCATGGAC CTGGAGGAGC CCGAAAAAGT GGACAAGCTG CAGGAGCCAC 1141 TGCTGGAAGC CCTGAGGCTG TACGCCCGGC GCCGGCGGCC CAGCCAGCCC TACATGTTCC 1201 CAAGGATGCT AATGAAAATC ACCGACCTCC GGGGCATCAG CACTAAGGGA GCTGAAAGGG 1261 CCATTACTCT GAAGATGGAG ATTCCAGGCC CGATGCCTCC CTTAATCCGA GAGATGCTGG 1321 AGAACCCTGA AATGTTTGAG GATGACTCCT CGCAGCCTGG TCCCCACCCC AATGCCTCTA 1381 GCGAGGATGA GGTTCCTGGG GGCCAGGGCA AAGGGGGCCT GAAGTCCCCA GCCTAGAACC 1441 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GAGAGCGTTC AAATGCTATA AACGGTACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt