Construct: ORF TRCN0000492183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001199.2_s317c1
- Derived from:
- ccsbBroadEn_06690
- DNA Barcode:
- TTGATGCCCTACTATAACCCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAX7 (5081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5081 | PAX7 | paired box 7 | NM_013945.3 | 99.9% | 100% | 873A>G |
| 2 | human | 5081 | PAX7 | paired box 7 | NM_002584.3 | 99.5% | 99.6% | 450_455delAGGTTT;879A>G |
| 3 | human | 5081 | PAX7 | paired box 7 | NM_001135254.2 | 90% | 88.3% | (many diffs) |
| 4 | mouse | 18509 | Pax7 | paired box 7 | NM_011039.2 | 83.4% | 86.3% | (many diffs) |
| 5 | mouse | 18509 | Pax7 | paired box 7 | XM_006538631.1 | 83.2% | 86.2% | (many diffs) |
| 6 | mouse | 18509 | Pax7 | paired box 7 | XM_006538629.1 | 83.1% | 86% | (many diffs) |
| 7 | mouse | 18509 | Pax7 | paired box 7 | XM_006538630.1 | 82.9% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1623
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccctt cccggcacgg taccgagaat gatgcggccg gctccggggc 121 agaactaccc ccgcacggga ttccctttgg aagtgtccac cccgcttggc caaggccggg 181 tcaatcagct gggaggggtc ttcatcaatg ggcgacccct gcctaaccac atccgccaca 241 agatagtgga gatggcccac catggcatcc ggccctgtgt catctcccga cagctgcgtg 301 tctcccacgg ctgcgtctcc aagattcttt gccgctacca ggagaccggg tccatccggc 361 ctggggccat cggcggcagc aagcccagac aggtggcgac tccggatgta gagaaaaaga 421 ttgaggagta caagagggaa aacccaggca tgttcagctg ggagatccgg gacaggctgc 481 tgaaggatgg gcactgtgac cgaagcactg tgccctcagt gagttcgatt agccgcgtgc 541 tcagaatcaa gttcgggaag aaagaggagg aggatgaagc ggacaagaag gaggacgacg 601 gcgaaaagaa ggccaaacac agcatcgacg gcatcctggg cgacaaaggg aaccggctgg 661 acgagggctc ggatgtggag tcggaacctg acctcccact gaagcgcaag cagcgacgca 721 gtcggaccac attcacggcc gagcagctgg aggagctgga gaaggccttt gagaggaccc 781 actacccaga catatacacc cgcgaggagc tggcgcagag gaccaagctg acagaggcgc 841 gtgtgcaggt ctggttcagt aaccgccgcg cccgttggcg taagcaggca ggagccaacc 901 agctggcggc gttcaaccac cttctgccag gaggcttccc gcccaccggc atgcccacgc 961 tgccccccta ccagctgccg gactccacct accccaccac caccatctcc caagatgggg 1021 gcagcactgt gcaccggcct cagcccctgc caccgtccac catgcaccag ggcgggctgg 1081 ctgcagcggc tgcagccgcc gacaccagct ctgcctacgg agcccgccac agcttctcca 1141 gctactctga cagcttcatg aatccggcgg cgccctccaa ccacatgaac ccggtcagca 1201 acggcctgtc tcctcaggtg atgagcaTCT TGGGCAACCC CAGTGCGGTG CCCCCGCAGC 1261 CACAGGCTGA CTTCTCCATC TCCCCGCTGC ATGGCGGCCT GGACTCGGCC ACCTCCATCT 1321 CAGCCAGCTG CAGCCAGCGG GCCGACTCCA TCAAGCCAGG AGACAGCCTG CCCACCTCCC 1381 AGGCCTACTG CCCACCCACC TACAGCACCA CCGGCTACAG CGTGGACCCC GTGGCCGGCT 1441 ATCAGTACGG CCAGTACGGC CAGAGTGAGT GCCTGGTGCC CTGGGCGTCC CCCGTCCCCA 1501 TTCCTTCTCC CACCCCCAGG GCCTCCTGCT TGTTTATGGA GAGCTACAAG GTGGTGTCAG 1561 GGTGGGGAAT GTCCATTTCA CAGATGGAAA AATTGAAGTC CAGCCAGATG GAACAGTTCA 1621 CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGATT GATGCCCTAC TATAACCCTT ACACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt