Construct: ORF TRCN0000492207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014626.2_s317c1
- Derived from:
- ccsbBroadEn_10505
- DNA Barcode:
- GAGGTCTCTGCGTGAGTACTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHA8P1 (51054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492207
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515596.2 | 82.2% | 80.8% | (many diffs) |
| 2 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012746.1 | 75.1% | 73.6% | (many diffs) |
| 3 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012747.1 | 75.1% | 73.6% | (many diffs) |
| 4 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001197026.2 | 73.9% | 72.8% | (many diffs) |
| 5 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001363474.1 | 71.5% | 66.7% | (many diffs) |
| 6 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515592.3 | 68.4% | 64.2% | (many diffs) |
| 7 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_011515591.2 | 68.4% | 64.6% | (many diffs) |
| 8 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_017012744.1 | 68.4% | 64.6% | (many diffs) |
| 9 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001197027.1 | 65.8% | 65% | (many diffs) |
| 10 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350973.2 | 65.6% | 65% | (many diffs) |
| 11 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | XM_005249897.2 | 65.5% | 61.7% | (many diffs) |
| 12 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350975.1 | 64.1% | 59.3% | (many diffs) |
| 13 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001350974.2 | 62.4% | 61.3% | (many diffs) |
| 14 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_032639.4 | 62.3% | 61.3% | (many diffs) |
| 15 | human | 84725 | PLEKHA8 | pleckstrin homology domain ... | NM_001363473.1 | 62.2% | 61.1% | (many diffs) |
| 16 | human | 51054 | PLEKHA8P1 | pleckstrin homology domain ... | NR_037144.1 | 62.1% | 1_537del;600G>A;1711_1886del | |
| 17 | mouse | 231999 | Plekha8 | pleckstrin homology domain ... | NM_001001335.2 | 71% | 73.4% | (many diffs) |
| 18 | mouse | 231999 | Plekha8 | pleckstrin homology domain ... | NM_001164361.1 | 64.9% | 67% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaactaaga ctctgctgtg acctccttgt tcagcaagta gataaaacaa 121 aagaagtaac cacaactggt gtgtccaatt ctgaggaggg aattgatgtg ggaactttgc 181 tgaaatcaac ctgtaatact tttctgaaga ccttggaaga atgcatgcag attgcaaatg 241 cagccttcac ctctgagctg ctctaccaca ctccaccagg atcaccacag ctggccatgc 301 tcaagtccag caagatgaaa catcctatta taccaattca taattcattg gaaaggcaaa 361 cggagttgag cacttgtgaa aatggatctt taaatatgga aataaatggt gaggaagaaa 421 tcctaatgaa aaataagaat tccttatatt tgaaatctgc agagatagac tgcagcatat 481 caagtgagga aaatacagat gataatataa ccgtccaagg tgaaataatg aaggaagata 541 gaatggaaaa cctgaaaaat catgacaata acttgtctca gtctggatca gactcaagtt 601 gctctccaga atgcctctgg gaggaaggca aagaagttat cccaactttc tttagtacca 661 tgaacacaag ctttagtgac attgaacttc tggaagacag tggcattccc acagaagcat 721 tcttggcatc atgttgtgct gtggttccag tattagacaa acttggccct acagtgtttg 781 ctcctgttaa gatggatctt gttgaaaata ttaagaaagt aaatcagaag tatataacca 841 aCAAAGAAGA GTTTACCACT CTCCAGAAGA TAGTGCTGCA CGAAGTGGAG GCGGATGTAG 901 CCCAGGTTAG GAACTCAGCG ACTGAAGCCC TCTTGTGGCT GAAGAGAGGT CTCAAATTTT 961 TGAAGGGATT TTTGACAGAA GTGAAAAATG GGGAAAAGGA TATCCAGACA GCCCTGAATA 1021 ACGCATATGG TAAAACATTG CGGCAACACC ATGGCTGGGT AGTTCGAGGG GTTTTTGCGT 1081 TAGCTTTAAG GGCAACTCCA TCCTATGAAG ATTTTGTGGC CGCGTTAACC GTAAAGGAAG 1141 GTGACCACCG GAAAGAAGCT TTCAGTATTG GGATGCAGAG GGACCTCAGC CTTTACCTCC 1201 CTGCCATGAA GAAGCAGATG GCCATACTGG ACGCTTTATA CCCAACTTTC TTGTACAAAG 1261 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1321 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1381 ACGAGAGGTC TCTGCGTGAG TACTCCCCAC GCGTTAAGTC gacaatcaac ctctggatta 1441 caaaatttgt gaaagatt