Construct: ORF TRCN0000492211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021122.2_s317c1
- DNA Barcode:
- TAGTACAGGGTCGCGACACTTACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CRY1 (1407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1407 | CRY1 | cryptochrome circadian regu... | NM_004075.5 | 99.9% | 100% | 636T>C |
| 2 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_017018832.2 | 93.8% | 91.3% | (many diffs) |
| 3 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448844.1 | 92.6% | 87.2% | 636T>C;1656_1729del;1833_1896del |
| 4 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448845.1 | 86.9% | 79.3% | (many diffs) |
| 5 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | NM_007771.3 | 86.3% | 93.3% | (many diffs) |
| 6 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243348.1 | 79% | 83.7% | (many diffs) |
| 7 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243349.1 | 62.2% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1830
- ORF length:
- 1758
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggggtg aacgccgtgc actggttccg aaaggggctc cggctccacg 121 acaaccccgc cctgaaggag tgcattcagg gcgccgacac catccgctgc gtctacatcc 181 tggacccctg gttcgccggc tcctccaatg tgggcatcaa caggtggcga tttttgcttc 241 agtgtcttga ggatcttgat gccaatctac gaaaattaaa ctcccgtctg tttgtgattc 301 gtggacaacc agcagatgtg tttcccaggc ttttcaagga atggaacatt actaaacttt 361 caattgagta tgattctgag ccctttggaa aggaacgaga cgcagctatt aagaaactgg 421 caactgaagc tggagtagaa gtcattgtaa gaatttcaca tacattatat gacctagaca 481 agatcataga actcaatggt ggacaaccgc ctctaactta taaaagattc cagactctca 541 tcagcaaaat ggaaccacta gagataccag tagagacaat tacttcagaa gtgatagaaa 601 agtgcacaac tcctctgtct gatgaccatg atgagaaata tggagtccct tcactggaag 661 agctaggttt tgatacagat ggcttatcct ctgcagtgtg gccaggcgga gaaactgaag 721 cacttactcg tttggaaagg catttggaaa gaaaagcttg ggtggcaaat tttgaaagac 781 ctcgaatgaa tgcgaattct ctgcttgcaa gccctactgg acttagtcct tatctccgat 841 ttggttgttt gtcatgtcga ctgttttact tcaaactaac agatctctac aaaaaggtaa 901 agaagaacag ttcccctccc ctttcccttt atgggcaact gttatggcgt gaatttttct 961 atacagcagc aacaaataat ccacgctttg ataaaatgga aggaaaccct atctgtgttc 1021 agattccttg ggataaaaat cctgaggctt tagccaaatg ggcggaaggc cggacaggct 1081 ttccatggat tgatgccatc atgacacagc ttcgtcagga gggttggatt catcatctag 1141 ccaggcatgc agttgcttgc ttcctgacac gaggggacct gtggattagt tgggaagaag 1201 gaatgaaggt atttgaagaa ttattgcttg atgcagattg gagcataaat gctggaagtt 1261 ggatgtggct gtcttgtagt tccttttttc aacagttttt tcactgctat tgccctgttg 1321 gttttggtag gagaacagat cccaatggag actatatcag gcgttatttg cctgtcctaa 1381 gaggcttccc tgcaaaatat atctatgatc cctggaatgc accagaaggt atccaaaagg 1441 tagccaaatg tttgatagga gttaattaTC CTAAACCAAT GGTGAACCAT GCTGAGGCAA 1501 GCCGTTTGAA TATCGAAAGG ATGAAACAGA TCTATCAGCA GCTTTCACGA TATAGAGGAC 1561 TAGGTCTTCT GGCATCAGTA CCTTCTAATC CTAATGGGAA TGGAGGCTTC ATGGGATATT 1621 CTGCAGAAAA TATCCCAGGT TGTAGCAGCA GTGGAAGTTG CTCTCAAGGG AGTGGTATTT 1681 TACACTATGC TCATGGCGAC AGTCAGCAAA CTCACCTGTT GAAGCAAGGA AGAAGCTCCA 1741 TGGGCACTGG TCTCAGTGGT GGGAAACGTC CTAGTCAGGA AGAGGACACA CAGAGTATTG 1801 GTCCTAAAGT CCAGAGACAG AGCACTAATT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1981 GTACAGGGTC GCGACACTTA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt