Construct: ORF TRCN0000492242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021347.2_s317c1
- DNA Barcode:
- CCTAGAACAACGAAGAACAGCATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KCNG1 (3755)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3755 | KCNG1 | potassium voltage-gated cha... | NM_002237.4 | 51.6% | 49.8% | (many diffs) |
| 2 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_006723785.3 | 51.6% | 49.8% | (many diffs) |
| 3 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528800.2 | 51.6% | 49.8% | (many diffs) |
| 4 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528801.2 | 51.6% | 49.8% | (many diffs) |
| 5 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528802.2 | 51.6% | 49.8% | (many diffs) |
| 6 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528803.2 | 51.6% | 49.8% | (many diffs) |
| 7 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528804.1 | 51.6% | 49.8% | (many diffs) |
| 8 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528805.2 | 51.6% | 49.8% | (many diffs) |
| 9 | human | 3755 | KCNG1 | potassium voltage-gated cha... | XM_011528806.2 | 51.6% | 49.8% | (many diffs) |
| 10 | mouse | 241794 | Kcng1 | potassium voltage-gated cha... | NM_001081134.1 | 44.8% | 44.2% | (many diffs) |
| 11 | mouse | 241794 | Kcng1 | potassium voltage-gated cha... | XM_006499538.3 | 44.8% | 44.2% | (many diffs) |
| 12 | mouse | 241794 | Kcng1 | potassium voltage-gated cha... | XM_006499539.3 | 44.8% | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 897
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaccctc ttaccgggag acaattctga ctacgactac agcgcgctga 121 gctgcacctc ggacgcctcc ttccacccgg ccttcctccc gcagcgccag gccatcaagg 181 gcgcgttcta ccgccgggcg cagcggctgc ggccgcagga tgagccccgc cagggctgtc 241 agcccgagga ccgccgccgt cggatcatca tcaacgtagg cggcatcaag tactcgctgc 301 cctggaccac gctggacgag ttcccgctga cgcgcctggg ccagctcaag gcctgcacca 361 acttcgacga catcctcaac gtgtgcgatg actacgacgt cacctgcaac gagttcttct 421 tcgaccgcaa cccgggggcc ttcggcacta tcctgacctt cctgcgcgcg ggcaagctgc 481 ggctgctgcg cgagatgtgc gcgctgtcct tcCAGGAGGA GCTGCTGTAC TGGGGCATCG 541 CGGAGGACCA CCTGGACGGC TGCTGCAAGC GCCGCTACCT GCAGAAGATT GAGGAGTTCG 601 CGGAGATGGT GGAGCGGGAG GAAGAGGACG ACGCGCTGGA CAGCGAGGGC CGCGACAGCG 661 AGGGCCCGGC CGAGGGCGAG GGCCGCCTGG GGCGCTGCAT GCGGCGACTG CGCGACATGG 721 TGGAGAGGCC GCACTCGGGG CTGCCTGGCA AGGTGTTCGC CTGCCTGTCG GTGCTCTTCG 781 TGACCGTCAC CGCCGTCAAC CTCTCCGTCA GCACCTTGCC CAGCCTGAGG GAGGAGGAGG 841 AGCAGGTAAG AGCCCACGCC CCGCGGGGAA ACGCGCCACC ACGAGGGAAG GGACTCTAGA 901 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 961 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1021 TTTATATATC TTGTGGAAAG GACGACCTAG AACAACGAAG AACAGCATCA CGCGTTAAGT 1081 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt