Transcript: Human XM_011528806.2

PREDICTED: Homo sapiens potassium voltage-gated channel modifier subfamily G member 1 (KCNG1), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KCNG1 (3755)
Length:
2418
CDS:
494..2035

Additional Resources:

NCBI RefSeq record:
XM_011528806.2
NBCI Gene record:
KCNG1 (3755)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011528806.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430949 GTGACATCTTGTTCGGAAGTG pLKO_005 1986 CDS 100% 4.050 5.670 N KCNG1 n/a
2 TRCN0000438927 ACAATAACTGAGCGCGGAGGA pLKO_005 2025 CDS 100% 2.160 3.024 N KCNG1 n/a
3 TRCN0000045111 CTCGGACACCAGAGACAATAA pLKO.1 2011 CDS 100% 13.200 9.240 N KCNG1 n/a
4 TRCN0000045112 CACCTGCAACGAGTTCTTCTT pLKO.1 823 CDS 100% 4.950 3.465 N KCNG1 n/a
5 TRCN0000445273 GAGCAAGAGAGGGTGATGTTC pLKO_005 1910 CDS 100% 4.950 3.465 N KCNG1 n/a
6 TRCN0000045110 GATGTGCCACAACGTCTTCAT pLKO.1 1282 CDS 100% 4.950 3.465 N KCNG1 n/a
7 TRCN0000441310 ATTGAGGAGTTCGCGGAGATG pLKO_005 1010 CDS 100% 4.050 2.835 N KCNG1 n/a
8 TRCN0000442170 CGTGAAAGGAAGCTGGGTCAT pLKO_005 2244 3UTR 100% 4.050 2.835 N KCNG1 n/a
9 TRCN0000442744 CTGTTCCCAGATGTGCCACAA pLKO_005 1273 CDS 100% 4.050 2.835 N KCNG1 n/a
10 TRCN0000421523 TACGTCATCGAGAACGAGATG pLKO_005 1679 CDS 100% 4.050 2.835 N KCNG1 n/a
11 TRCN0000045108 CGTCGGATCATCATCAACGTA pLKO.1 680 CDS 100% 3.000 2.100 N KCNG1 n/a
12 TRCN0000428497 GAACACTAGAACATCAGCAGA pLKO_005 2198 3UTR 100% 2.640 1.848 N KCNG1 n/a
13 TRCN0000045109 CCAGGACAGTGACATCTTGTT pLKO.1 1978 CDS 100% 4.950 2.970 N KCNG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011528806.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10933 pDONR223 100% 51.6% 49.8% None (many diffs) n/a
2 ccsbBroad304_10933 pLX_304 0% 51.6% 49.8% V5 (many diffs) n/a
3 TRCN0000480643 CTGGCTGCAGCAATGCAAGATCGT pLX_317 52.6% 51.6% 49.8% V5 (many diffs) n/a
4 TRCN0000492242 CCTAGAACAACGAAGAACAGCATC pLX_317 46.6% 51.6% 49.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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