Construct: ORF TRCN0000492255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021525.2_s317c1
- DNA Barcode:
- CGGCCTCCTGGCAGCAATACTTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIF2C (11004)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11004 | KIF2C | kinesin family member 2C | NM_006845.4 | 100% | 100% | |
| 2 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297655.2 | 94.3% | 94.2% | 439_440ins123 |
| 3 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297656.2 | 92.4% | 92.4% | 0_1ins163;2delT |
| 4 | human | 11004 | KIF2C | kinesin family member 2C | NM_001297657.2 | 84.4% | 84.4% | 0_1ins339 |
| 5 | human | 11004 | KIF2C | kinesin family member 2C | XM_011540540.2 | 84.4% | 84.4% | 0_1ins339 |
| 6 | human | 11004 | KIF2C | kinesin family member 2C | XM_011540541.2 | 84.4% | 84.4% | 0_1ins339 |
| 7 | mouse | 73804 | Kif2c | kinesin family member 2C | NM_134471.4 | 86.2% | 87% | (many diffs) |
| 8 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_006503433.2 | 80.5% | 81.4% | (many diffs) |
| 9 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240620.2 | 80.5% | 81.4% | (many diffs) |
| 10 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240621.2 | 80.5% | 81.4% | (many diffs) |
| 11 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_017320411.1 | 80.5% | 81.4% | (many diffs) |
| 12 | mouse | 73804 | Kif2c | kinesin family member 2C | NM_001290662.1 | 80.2% | 81.4% | (many diffs) |
| 13 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_006503435.3 | 80.2% | 81.4% | (many diffs) |
| 14 | mouse | 73804 | Kif2c | kinesin family member 2C | XM_011240622.2 | 80.2% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2244
- ORF length:
- 2175
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccatggac tcgtcgcttc aggcccgcct gtttcccggt ctcgctatca 121 agatccaacg cagtaatggt ttaattcaca gtgccaatgt aaggactgtg aacttggaga 181 aatcctgtgt ttcagtggaa tgggcagaag gaggtgccac aaagggcaaa gagattgatt 241 ttgatgatgt ggctgcaata aacccagaac tcttacagct tcttccctta catccgaagg 301 acaatctgcc cttgcaggaa aatgtaacaa tccagaaaca aaaacggaga tccgtcaact 361 ccaaaattcc tgctccaaaa gaaagtcttc gaagccgctc cactcgcatg tccactgtct 421 cagagcttcg catcacggct caggagaatg acatggaggt ggagctgcct gcagctgcaa 481 actcccgcaa gcagttttca gttcctcctg cccccactag gccttcctgc cctgcagtgg 541 ctgaaatacc attgaggatg gtcagcgagg agatggaaga gcaagtccat tccatccgag 601 gcagctcttc tgcaaaccct gtgaactcag ttcggaggaa atcatgtctt gtgaaggaag 661 tggaaaaaat gaagaacaag cgagaagaga agaaggccca gaactctgaa atgagaatga 721 agagagctca ggagtatgac agtagttttc caaactggga atttgcccga atgattaaag 781 aatttcgggc tactttggaa tgtcatccac ttactatgac tgatcctatc gaagagcaca 841 gaatatgtgt ctgtgttagg aaacgcccac tgaataagca agaattggcc aagaaagaaa 901 ttgatgtgat ttccattcct agcaagtgtc tcctcttggt acatgaaccc aagttgaaag 961 tggacttaac aaagtatctg gagaaccaag cattctgctt tgactttgca tttgatgaaa 1021 cagcttcgaa tgaagttgtc tacaggttca cagcaaggcc actggtacag acaatctttg 1081 aaggtggaaa agcaacttgt tttgcatatg gccagacagg aagtggcaag acacatacta 1141 tgggcggaga cctctctggg aaagcccaga atgcatccaa agggatctat gccatggcct 1201 cccgggacgt cttcctcctg aagaatcaac cctgctaccg gaagttgggc ctggaagtct 1261 atgtgacatt cttcgagatc tacaatggga agctgtttga cctgctcaac aagaaggcca 1321 agctgcgcgt gctggaggac ggcaagcaac aggtgcaagt ggtggggctg caggagcatc 1381 tggttaactc tgctgatgat gtcatcaaga tgatcgacat gggcagcgcc tgcagaacct 1441 ctgggcagac atttgccaac tccaattcct cccgctccca cgcgtgcttc caaattattc 1501 ttcgagctaa agggagaatg catggcaagt tctctttggt agatctggca gggaatgagc 1561 gaggcgcgga cacttccagt gctgaccggc agacccgcat ggagggcgca gaaatcaaca 1621 agagtctctt agccctgaag gagtgcatca gggccctggg acagaacaag gctcacaccc 1681 cgttccgtga gagcaagctg acacaggtgc tgagggactc cttcattggg gagaactcta 1741 ggacttgcat gattgccacg atctcaccag gcataagctc ctgtgaatat actttaaaca 1801 ccctgagata tgcagacagg gtcaaggagc tgagccccca cagtgggccc agtggagagc 1861 agttgattca aatggaaaca gaagagatgg aagcctgctc taacggggcg ctgattccag 1921 gcaatttatc caaggaagag gaggaactgt cttcccagat gtccagcttt aacgaagcca 1981 tgactcagat cagggagctg gaggagaagg ctatggaaga gctcaaggag atcatacagc 2041 aaggaccaga ctggcttgag ctctctgaga tgaccgagca gccagactat gacctggaga 2101 cctttgtgaa caaagcggaa tctgctctgg cccagcaagc caagcatttc tcagCCCTGC 2161 GAGATGTCAT CAAGGCCTTG CGCCTGGCCA TGCAGCTGGA AGAGCAGGCT AGCAGACAAA 2221 TAAGCAGCAA GAAACGGCCC CAGTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGGCCTCC 2401 TGGCAGCAAT ACTTCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt