Construct: ORF TRCN0000492258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000492.1_s317c1
- Derived from:
- ccsbBroadEn_15679
- DNA Barcode:
- ATCCCCAGCCGATTGCACGGGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX38 (9785)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9785 | DHX38 | DEAH-box helicase 38 | NM_014003.4 | 100% | 100% | |
| 2 | human | 9785 | DHX38 | DEAH-box helicase 38 | XM_011523484.2 | 100% | 100% | |
| 3 | human | 9785 | DHX38 | DEAH-box helicase 38 | XM_011523485.1 | 100% | 100% | |
| 4 | human | 9785 | DHX38 | DEAH-box helicase 38 | XM_005256269.2 | 97.1% | 97.1% | 509_510ins105 |
| 5 | human | 9785 | DHX38 | DEAH-box helicase 38 | XM_017023913.2 | 97.1% | 97.1% | 509_510ins105 |
| 6 | human | 9785 | DHX38 | DEAH-box helicase 38 | XR_429741.3 | 85.8% | 1_176del;2760_2761insCAGGTCAGTGTTT;3845_4262del | |
| 7 | mouse | 64340 | Dhx38 | DEAH (Asp-Glu-Ala-His) box ... | XM_006531261.3 | 90.2% | 97.3% | (many diffs) |
| 8 | mouse | 64340 | Dhx38 | DEAH (Asp-Glu-Ala-His) box ... | NM_178380.2 | 90.1% | 97.2% | (many diffs) |
| 9 | mouse | 64340 | Dhx38 | DEAH (Asp-Glu-Ala-His) box ... | NR_151644.1 | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3747
- ORF length:
- 3681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacaccagt gaggatgcct cgatccatcg attggaaggc actgatctgg 121 actgtcaggt tggtggtctt atttgcaagt ccaaaagtgc ggccagcgag cagcatgtct 181 tcaaggctcc tgctccccgc ccttcattac tcggactgga cttgctggct tccctgaaac 241 ggagagagcg agaggagaag gacgatgggg aggacaagaa gaagtccaaa gtctcctcct 301 acaaggactg ggaagagagc aaggatgacc agaaggatgc tgaggaagag ggcggtgacc 361 aggctggcca aaatatccgg aaagacagac attatcggtc tgctcgggta gagactccat 421 cccatccggg tggtgtgagc gaagagtttt gggaacgcag tcggcagaga gagcgggagc 481 ggcgggaaca tggtgtctat gcctcgtcca aagaagaaaa ggattggaag aaggagaaat 541 cgcgggatcg agactatgac cgcaagaggg acagagatga gcgggataga agtaggcaca 601 gcagcagatc agagcgagat ggagggtcag agcgtagcag cagaagaaat gaacccgaga 661 gcccacgaca tcgacctaaa gatgcagcca ccccttcaag gtctacctgg gaggaagagg 721 acagtggcta tggctcctca aggcgctcac agtgggaatc gccctccccg acgccttcct 781 atcgggattc tgagcggagc catcggctgt ccactcgaga tcgagacagg tctgtgaggg 841 gcaagtactc ggatgacacg cctctgccaa ctccctccta caaatataac gagtgggccg 901 atgacagaag acacttgggg tccaccccgc gtctgtccag gggccgagga agacgtgagg 961 agggcgaaga aggaatttca tttgacacgg aggaggagcg gcagcagtgg gaagatgacc 1021 agaggcaagc cgatcgggat tggtacatga tggacgaggg ctatgacgag ttccacaacc 1081 cgctggccta ctcctccgag gactacgtga ggaggcggga gcagcacctg cataaacaga 1141 agcagaagcg catttcagct cagcggagac agatcaatga ggataacgag cgctgggaga 1201 caaaccgcat gctcaccagt ggggtggtcc atcggctgga ggtggatgag gactttgaag 1261 aggacaacgc ggccaaggtg catctgatgg tgcacaatct ggtgcctccc tttctggatg 1321 ggcgcattgt cttcaccaag cagccggagc cggtgattcc agtgaaggat gctacttctg 1381 acctggccat cattgctcgg aaaggcagcc agacagtgcg gaagcacagg gagcagaagg 1441 agcgcaagaa ggctcagcac aaacactggg aactggcggg gaccaaactg ggagatataa 1501 tgggcgtcaa gaaggaggaa gagccagata aagctgtgac ggaggatggg aaggtggact 1561 acaggacaga gcagaagttt gcagatcaca tgaagagaaa gagcgaagcc agcagtgaat 1621 ttgcaaagaa gaagtccatc ctggagcaga ggcagtacct gcccatcttt gcagtgcagc 1681 aggagctgct cactattatc agagacaaca gcatcgtgat cgtggttggg gagacgggga 1741 gtggtaagac cactcagctg acgcagtacc tgcatgaaga tggttacacg gactatggga 1801 tgattgggtg tacccagccc cggcgtgtag ctgccatgtc agtggccaag agagtcagtg 1861 aagagatggg gggaaacctt ggcgaggagg tgggctatgc catccgcttt gaagactgca 1921 cttcagagaa caccttgatc aaatacatga ctgacgggat cctgctccga gagtccctcc 1981 gggaagccga cctggatcac tacagtgcca tcatcatgga cgaggcccac gagcgctccc 2041 tcaacactga cgtgctcttt gggctgctcc gggaggtagt ggctcggcgc tcagacctga 2101 agctcatcgt cacatcagcc acgatggatg cggagaagtt tgctgccttt tttgggaatg 2161 tccccatctt ccacatccct ggccgtacct tccctgttga catcctcttc agcaagaccc 2221 cacaggagga ttacgtggag gctgcagtga agcagtcctt gcaggtgcac ctgtcggggg 2281 cccctggaga catccttatc ttcatgcctg gccaagagga cattgaggtg acctcagacc 2341 agattgtgga gcatctggag gaactggaga acgcgcctgc cctggctgtg ctgcccatct 2401 actctcagct gccttctgac ctccaggcca aaatcttcca gaaggctcca gatggcgttc 2461 ggaagtgcat cgttgccacc aatattgccg agacgtctct cactgttgac ggcatcatgt 2521 ttgttatcga ttctggttat tgcaaattaa aggtcttcaa ccccaggatt ggcatggatg 2581 ctctgcagat ctatcccatt agccaggcca atgccaacca gcggtcaggg cgagccggca 2641 ggacgggccc aggtcagtgt ttcaggctct acacccagag cgcctacaag aatgagctcc 2701 tgaccaccac agtgcccgag atccagagga ctaacctggc caacgtggtg ctgctgctca 2761 agtccctcgg ggtgcaggac ctgctgcagt tccacttcat ggacccgccc ccggaggaca 2821 acatgctcaa ctctatgtat cagctctgga tcctcggggc cctggacaac acaggtggtc 2881 tgacctctac cgggcggctg atggtggagt tcccgctgga ccctgccctg tccaagatgc 2941 tcatcgtgtc ctgtgacatg ggctgcagct ccgagatcct gctcatcgtt tccatgctct 3001 cggtcccagc catcttctac aggcccaagg gtcgagagga ggagagtgat caaatccggg 3061 agaagttcgc tgttcctgag agcgatcatt tgacctacct gaatgtttac ctgcagtgga 3121 agaacaataa ttactccacc atctggtgta acgatcattt catccatgct aaggccatgc 3181 ggaaggtccg ggaggtgcga gctcaactca aggacatcat ggtgcagcag cggatgagcc 3241 tggcctcgtg tggcactgac tgggacatcg tcaggaagtg catctgtgct gcctatttcc 3301 accaagcagc caagctcaag ggaatcgggg agtacgtgaa catccgcaca gggatgcccT 3361 GCCACTTGCA CCCCACCAGC TCCCTTTTTG GAATGGGCTA CACCCCAGAT TACATAGTGT 3421 ATCACGAGTT GGTCATGACC ACCAAGGAGT ATATGCAGTG TGTGACCGCT GTGGACGGGG 3481 AGTGGCTGGC GGAGCTGGGC CCCATGTTCT ATAGCGTGAA ACAGGCGGGC AAGTCACGGC 3541 AGGAGAACCG TCGTCGGGCC AAAGAGGAAG CCTCTGCCAT GGAGGAGGAG ATGGCGCTGG 3601 CCGAGGAGCA GCTGCGAGCC CGGCGGCAGG AGCAGGAGAA GCGCAGCCCC CTGGGCAGTG 3661 TCAGGTCTAC GAAGATCTAC ACTCCAGGCC GGAAAGAGCA AGGGGAGCCC ATGACCCCTC 3721 GCCGCACGCC AGCCCGCTTT GGTCTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 3781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCCCCAG 3901 CCGATTGCAC GGGCTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3961 aagatt