Construct: ORF TRCN0000492260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011630.1_s317c1
- Derived from:
- ccsbBroadEn_10927
- DNA Barcode:
- CACAAAAAGGGCTAGGCCAAGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGB8 (3696)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3696 | ITGB8 | integrin subunit beta 8 | NM_002214.3 | 56.7% | 55.3% | (many diffs) |
| 2 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012178.1 | 56.7% | 55.3% | (many diffs) |
| 3 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012179.1 | 56.7% | 55.3% | (many diffs) |
| 4 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_011515394.2 | 52.9% | 50.5% | (many diffs) |
| 5 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_011515393.2 | 52.7% | 50.8% | (many diffs) |
| 6 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_011515396.1 | 39.2% | 37.9% | (many diffs) |
| 7 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012180.1 | 39.2% | 37.9% | (many diffs) |
| 8 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012181.1 | 39.2% | 37.9% | (many diffs) |
| 9 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012182.1 | 39.2% | 37.9% | (many diffs) |
| 10 | human | 3696 | ITGB8 | integrin subunit beta 8 | XM_017012183.1 | 39.2% | 37.9% | (many diffs) |
| 11 | mouse | 320910 | Itgb8 | integrin beta 8 | NM_177290.3 | 50% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cggctcggcc ctggcttttt ttaccgctgc atttgtctgc ctgcaaaacg 121 accggcgagg tcccgcctcg ttcctctggg cagcctgggt gttttcactt gttcttggac 181 tgggccaagg tgaagacaat agatgtgcat cttcaaatgc agcatcctgt gccaggtgcc 241 ttgcgctggg tccagaatgt ggatggtgtg ttcaagagga tttcatttca ggtggatcaa 301 gaagtgaacg ttgtgatatt gtttccaatt taataagcaa aggctgctca gttgattcaa 361 tagaataccc atctgtgcat gttataatac ccactgaaaa tgaaattaat acccaggtga 421 caccaggaga agtgtctatc cagctgcgtc caggagccga agctaatttt atgctgaaag 481 ttcatcctct gaagaaatat cctgtggatc tttattatct tgttgatgtc tcagcatcaa 541 tgcacaataa tatagaaaaa ttaaattccg ttggaaacga tttatctaga aaaatggcat 601 ttttctcccg tgactttcgt cttggatttg gctcatacgt tgataaaaca gtttcaccat 661 acattagcat ccaccccgaa aggattcata atcaatgcag tgactacaat ttagactgca 721 tgcctcccca tggatacatc catgtgctgt ctttgacaga gaacatcact gagtttgaga 781 aagcagttca tagacagaag atctctggaa acatagatac accagaagga ggttttgacg 841 ccatgcttca ggcagctgtc tgtgaaagtc atatcggatg gcgaaaagag gctaaaagat 901 tgctgctggt gatgacagaT CAGACGTCTC ATCTCGCTCT TGATAGCAAA TTGGCAGGCA 961 TAGTGGTGCC CAATGACGGA AACTGTCATC TGAAAAACAA CGTCTATGTC AAATCGACAA 1021 CCATGGAACA CCCCTCACTA GGCCAACTTT CAGAGAAATT AATAGACAAC AACATTAATG 1081 TCATCTTTGC AGTTCAAGGA AAACAATTTC ATTGGTATAA GGATCTTCTA CCCCTCTTGC 1141 CAGGCACCAT TGCTGGTGAA ATAGAATCAA AGGCTGCAAA CCTCAATAAT TTGGTAGTGG 1201 AAGCCTATCA GAAGCTCATT TCAGAAGTGA AAGTTCAGGT GGAAAACCAG GTACAAGGCA 1261 TCTATTTTAA CATTACCGCC ATCTGTCCAG ATGGGTCCAG AAAGCCAGGC ATGGAAGGAT 1321 GCAGAAACGT GACGAGCAAT GATGAAGTAT GTGGGTGTGC ATTTTTCCCT TTTAAAATAA 1381 ACTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGACA CAAAAAGGGC TAGGCCAAGA TAACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt