Construct: ORF TRCN0000492274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020137.2_s317c1
- DNA Barcode:
- AGCTCCAGGAGATTATGGCGGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AK3 (50808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50808 | AK3 | adenylate kinase 3 | NM_016282.4 | 100% | 100% | |
| 2 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199852.2 | 82.3% | 82.3% | 150_151ins120 |
| 3 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199853.1 | 69.1% | 69.1% | 0_1ins210 |
| 4 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199855.1 | 69.1% | 69.1% | 0_1ins210 |
| 5 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199856.1 | 69.1% | 69.1% | 0_1ins210 |
| 6 | mouse | 56248 | Ak3 | adenylate kinase 3 | NM_021299.1 | 88.8% | 92.5% | (many diffs) |
| 7 | mouse | 56248 | Ak3 | adenylate kinase 3 | XM_006527222.3 | 71.7% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 753
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggggcg tccgcgcggc tgctgcgagc ggtgatcatg ggggccccgg 121 gctcgggcaa gggcaccgtg tcgtcgcgca tcactacaca cttcgagctg aagcacctct 181 ccagcgggga cctgctccgg gacaacatgc tgcggggcac agaaattggc gtgttagcca 241 aggctttcat tgaccaaggg aaactcatcc cagatgatgt catgactcgg ctggcccttC 301 ATGAGCTGAA AAATCTCACC CAGTATAGCT GGCTGTTGGA TGGTTTTCCA AGGACACTTC 361 CACAGGCAGA AGCCCTAGAT AGAGCTTATC AGATCGACAC AGTGATTAAC CTGAATGTGC 421 CCTTTGAGGT CATTAAACAA CGCCTTACTG CTCGCTGGAT TCATCCCGCC AGTGGCCGAG 481 TCTATAACAT TGAATTCAAC CCTCCCAAAA CTGTGGGCAT TGATGACCTG ACTGGGGAGC 541 CTCTCATTCA GCGTGAGGAT GATAAACCAG AGACGGTTAT CAAGAGACTA AAGGCTTATG 601 AAGACCAAAC AAAGCCAGTC CTGGAATATT ACCAGAAAAA AGGGGTGCTG GAAACATTCT 661 CCGGAACAGA AACCAACAAG ATTTGGCCCT ATGTATATGC TTTCCTACAA ACTAAAGTTC 721 CACAAAGAAG CCAGAAAGCT TCAGTTACTC CATGAGACCC AGCTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 AAGCTCCAGG AGATTATGGC GGCACACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt