Construct: ORF TRCN0000492289

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001986.1_s317c1
Derived from:
ccsbBroadEn_13277
DNA Barcode:
GGCCCTCACCACCCCGAGTGTCTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
RMDN2 (151393)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492289
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151393 RMDN2 regulator of microtubule dy... NM_001170791.3 96.8% 96.8% 1_39del
2 human 151393 RMDN2 regulator of microtubule dy... NM_001170792.2 96.8% 96.8% 1_39del
3 human 151393 RMDN2 regulator of microtubule dy... NM_001322211.1 96.8% 96.8% 1_39del
4 human 151393 RMDN2 regulator of microtubule dy... XM_017003478.1 95.5% 95.6% 1_39del;1230_1245delinsG
5 human 151393 RMDN2 regulator of microtubule dy... NM_001322212.2 93% 92.6% (many diffs)
6 human 151393 RMDN2 regulator of microtubule dy... XM_017003476.2 90.7% 90.6% 1_39del;1230_1311delinsG
7 human 151393 RMDN2 regulator of microtubule dy... XM_017003477.2 90.7% 90.6% 1_39del;1230_1311delinsG
8 human 151393 RMDN2 regulator of microtubule dy... XM_011532616.2 89% 87.2% (many diffs)
9 human 151393 RMDN2 regulator of microtubule dy... XM_011532617.2 89% 87.2% (many diffs)
10 human 151393 RMDN2 regulator of microtubule dy... XM_011532618.2 89% 87.2% (many diffs)
11 human 151393 RMDN2 regulator of microtubule dy... XM_011532619.2 89% 87.2% (many diffs)
12 human 151393 RMDN2 regulator of microtubule dy... NM_001170793.2 66.2% 64.8% (many diffs)
13 human 151393 RMDN2 regulator of microtubule dy... XM_017003479.1 60.3% 57.1% (many diffs)
14 human 151393 RMDN2 regulator of microtubule dy... XM_017003474.1 57.7% 46.6% (many diffs)
15 human 151393 RMDN2 regulator of microtubule dy... NM_144713.5 55.7% 44.2% (many diffs)
16 human 151393 RMDN2 regulator of microtubule dy... XM_017003475.2 55.7% 44.2% (many diffs)
17 human 151393 RMDN2 regulator of microtubule dy... XM_011532615.3 55.7% 44.8% (many diffs)
18 human 151393 RMDN2 regulator of microtubule dy... XM_011532613.3 54.6% 42.6% (many diffs)
19 human 151393 RMDN2 regulator of microtubule dy... XM_011532614.3 54.6% 42.6% (many diffs)
20 human 151393 RMDN2 regulator of microtubule dy... XR_939668.3 23.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1257
ORF length:
1191
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gggcactgct ggaatcagct tgctgctctt gtggtaccac aaggtccgta
121aaccagggat agcaatgaag ttacctgaat ttctttctct gggtaataca tttaattcaa
181taactttgca agatgaaata catgatgacc aaggaacaac agtaatcttt caagaaaggc
241aacttcagat actggagaag ttaaacgaat tactgacaaa tatggaagaa ctcaaagagg
301aaatcagatt tcttaaagaa gctattccaa agctggagga atatatacaa gatgaacttg
361gagggaaaat aactgttcat aagataagcc ctcagcacag agcgagaaaa agaagactcc
421ccacaattca aagttcagca acaagtaata gttcagagga agcagaaagt gaaggagggt
481atattacagc taatactgac acagaagaac agagttttcc agtccctaag gcatttaaca
541cacgtgtaga ggaattaaat ttagatgtcc ttcttcagaa ggtagatcat ttacgtatga
601gtgagtctgg caagtcggag agttttgaac tacttcgtga ccacaaagaa aagtttagag
661atgaaataga gtttatgtgg cgatttgctc gtgcttatgg agacatgtat gaactatcta
721caaacacaca agaaaagaaa cattatgcta atattggaaa aactttaagt gaaagagcta
781ttaatagagc acccatgaat ggacattgtc atctgtggta tgcagttttg tgtggctatg
841tatctgagtt tgagggttta caaaacaaaa TCAACTATGG GCACCTCTTC AAGGAACATC
901TAGATATAGC AATCAAACTT TTACCAGAGG AACCCTTTCT ATATTACCTC AAAGGGAGAT
961ACTGCTATAC TGTCTCAAAA CTGAGCTGGA TTGAGAAAAA AATGGCTGCT ACTCTGTTTG
1021GAAAAATACC ATCTTCAACT GTACAAGAAG CTTTACACAA TTTCCTTAAG GCTGAAGAAC
1081TATGCCCTGG TTATTCTAAT CCCAATTACA TGTACTTAGC AAAGTGTTAT ACTGATCTTG
1141AGGAAAACCA GAATGCTTTG AAGTTCTGTA ATTTGGCTTT ATTGCTTCCT ACTGTTACCA
1201AAGAGGATAA AGAGGCACAG AAAGAGATGC AAAAAATAAT GACTTCCTTG AAGAGGTAAC
1261CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1321TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1381ATATATCTTG TGGAAAGGAC GAGGCCCTCA CCACCCCGAG TGTCTAACGC GTTAAGTCga
1441caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)