Construct: ORF TRCN0000492299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021818.2_s317c1
- DNA Barcode:
- TTCTAATTTCTGACTATTCGACAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A8 (23428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_012244.4 | 99.8% | 100% | 1146C>T;1170T>C |
| 2 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_001267036.1 | 76.4% | 71.7% | (many diffs) |
| 3 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_182728.3 | 61.9% | 62% | 0_1ins609;537C>T;561T>C |
| 4 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_001267037.1 | 58% | 52.2% | (many diffs) |
| 5 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | NM_016972.2 | 88.7% | 91.9% | (many diffs) |
| 6 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | XM_006519250.2 | 55.2% | 56.4% | (many diffs) |
| 7 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | XM_011245114.1 | 51.7% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1674
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaagaagga gccaggcacc gaaacaacac cgaaaagaaa cacccaggtg 121 ggggcgagtc ggacgccagc cccgaggctg gttccggagg gggcggagta gccctgaaga 181 aagagatcgg attggtcagt gcctgtggta tcatcgtagg gaacatcatc ggctctggaa 241 tctttgtctc gccaaaggga gtgctggaga atgctggttc tgtgggcctt gctctcatcg 301 tctggattgt gacgggcttc atcacagttg tgggagccct ctgctatgct gaactcgggg 361 tcaccatccc caaatctgga ggtgactact cctatgtcaa ggacatcttc ggaggactgg 421 ctgggttcct gaggctgtgg attgctgtgc tggtgatcta ccccaccaac caggctgtca 481 tcgccctcac cttctccaac tacgtgctgc agccgctctt ccccacctgc ttccccccag 541 agtctggcct tcggctcctg gctgccatct gcttattgct cctcacatgg gtcaactgtt 601 ccagtgtgcg gtgggccacc cgggttcaag acatcttcac agctgggaag ctcctggcct 661 tggccctgat tatcatcatg gggattgtac agatatgcaa aggagagtac ttctggctgg 721 agccaaagaa tgcatttgag aatttccagg aacctgacat cggcctcgtc gcactggctt 781 tccttcaggg ctcctttgcc tatggaggct ggaactttct gaattacgtg actgaggagc 841 ttgttgatcc ctacaagaac cttcccagag ccatcttcat ctccatccca ctggtcacat 901 ttgtgtatgt ctttgccaat gtcgcttatg tcactgcaat gtccccccag gagctgctgg 961 catccaacgc cgtcgctgtg acttttggag agaagctcct aggagtcatg gcctggatca 1021 tgcccatttc tgttgccctg tccacatttg gaggagttaa tgggtctctc ttcacctcct 1081 ctcggctgtt cttcgctgga gcccgagagg gccaccttcc cagtgtgttg gccatgatcc 1141 acgtgaagcg ctgcacccca atcccagccc tgctcttcac atgcatctcc accctgctga 1201 tgctggtcac cagtgacatg tacacactca tcaactacgt gggcttcatc aactacctct 1261 tctatggggt cacggttgct ggacagatag tccttcgctg gaagaagcct gatatccccc 1321 gccccatcaa gatcaacctg ctgttcccca tcatctactt gctgttctgg gccttcctgc 1381 tggtcttcag cctgtggtca gagccggtgg tgtgtggcat tggcctggcc atcatgctga 1441 caggagtgcc tgtctatttc ctgggtgttt actGGCAACA CAAGCCCAAG TGTTTCAGTG 1501 ACTTCATTGA GCTGCTAACC CTGGTGAGCC AGAAGATGTG TGTGGTCGTG TACCCCGAGG 1561 TGGAGCGGGG CTCAGGGACA GAGGAGGCTA ATGAGGACAT GGAGGAGCAG CAGCAGCCCA 1621 TGTACCAACC CACTCCCACG AAGGACAAGG ACGTGGCGGG GCAGCCCCAG CCCTAGAACC 1681 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GATTCTAATT TCTGACTATT CGACAAACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt